TRIAGEM METABÓLICA POR PKS E NRPS EM ACTINOBACTÉRIAS ENDOFÍTICAS DE Citrus reticulata

Metabolic screening for PKS and NRPS in endophytic actinobacteria from citrus reticulata

dc.creatorPedro L. R. da
dc.creatorCruz
dc.creatorLeila R.
dc.creatorGiarola
dc.creatorSuellen da Silva
dc.creatorMoraes
dc.creatorDéborah Ellen S. G. da
dc.creatorSilva
dc.creatorJoelma
dc.creatorMarcon
dc.creatorJoão L.
dc.creatorAzevedo
dc.creatorWelington L.
dc.creatorAraújo
dc.creatorLuciana G. de
dc.creatorOliveira,
dc.date2015
dc.date2016-06-07T19:30:44Z
dc.date2016-06-07T19:30:44Z
dc.date.accessioned2018-03-29T01:52:26Z
dc.date.available2018-03-29T01:52:26Z
dc.identifierTriagem MetabÓlica Por Pks E Nrps Em ActinobactÉrias EndofÍticas De Citrus Reticulata, v. 38, n.3, p. 333-341. 2015.
dc.identifier1678-7064
dc.identifierS0100-40422015000300333-scl
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000300333&lang=pt
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/244457
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1308155
dc.descriptionPolyketides and non-ribosomal peptides are natural products widely found in bacteria, fungi and plants. The biological activities associated with these metabolites have attracted special attention in biopharmaceutical studies. Polyketide synthases act similarly to fatty acids synthetases and the whole multi-enzymatic set coordinating precursor and extending unit selection and reduction levels during chain growth. Acting in a similarly orchestrated model, non-ribosomal peptide synthetases biosynthesize NRPs. PKSs-I and NRPSs enzymatic modules and domains are collinearly organized with the parent gene sequence. This arrangement allows the use of degenerated PCR primers to amplify targeted regions in the genes corresponding to specific enzymatic domains such as ketosynthases and acyltransferases in PKSs and adenilation domains in NRPSs. Careful analysis of these short regions allows the classifying of a set of organisms according to their potential to biosynthesize PKs and NRPs. In this work, the biosynthetic potential of a set of 13 endophytic actinobacteria from Citrus reticulata for producing PKs and NRP metabolites was evaluated. The biosynthetic profile was compared to antimicrobial activity. Based on the inhibition promoted, 4 strains were considered for cluster analysis. A PKS/NRPS phylogeny was generated in order to classify some of the representative sequences throughout comparison with homologous genes. Using this approach, a molecular fingerprint was generated to help guide future studies on the most promising strains.
dc.descriptionv. 38
dc.descriptionn.3
dc.description333
dc.description341
dc.languagePortuguês
dc.relationQuímica Nova
dc.rightsaberto
dc.sourceSciELO
dc.subjectEndophytic Actinobacteria
dc.subjectMolecular Fingerprint
dc.subjectPolyketides
dc.subjectNon-ribosomal Peptides
dc.subjectGenome Mining
dc.titleTRIAGEM METABÓLICA POR PKS E NRPS EM ACTINOBACTÉRIAS ENDOFÍTICAS DE Citrus reticulata
dc.titleMetabolic screening for PKS and NRPS in endophytic actinobacteria from citrus reticulata
dc.typeArtículos de revistas


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