Urinary Bladder Dysfunction In Transgenic Sickle Cell Disease Mice

dc.creatorClaudino
dc.creatorMario Angelo; Silveira Leiria
dc.creatorLuiz Osorio; da Silva
dc.creatorFabio Henrique; Alexandre
dc.creatorEduardo Costa; Renno
dc.creatorAndre; Monica
dc.creatorFabiola Zakia; de Nucci
dc.creatorGilberto; Fertrin
dc.creatorKleber Yotsumoto; Antunes
dc.creatorEdson; Costa
dc.creatorFernando Ferreira; Franco-Penteado
dc.creatorCarla Fernanda
dc.date2015-AUG
dc.date2016-06-07T13:35:00Z
dc.date2016-06-07T13:35:00Z
dc.date.accessioned2018-03-29T01:50:33Z
dc.date.available2018-03-29T01:50:33Z
dc.identifier
dc.identifierUrinary Bladder Dysfunction In Transgenic Sickle Cell Disease Mice. Public Library Science, v. 10, p. AUG-2015.
dc.identifier1932-6203
dc.identifierWOS:000358942700031
dc.identifier10.1371/journal.pone.0133996
dc.identifierhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133996
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/244026
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1307724
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionBackground Urological complications associated with sickle cell disease (SCD), include nocturia, enuresis, urinary infections and urinary incontinence. However, scientific evidence to ascertain the underlying cause of the lower urinary tract symptoms in SCD is lacking. Objective Thus, the aim of this study was to evaluate urinary function, in vivo and ex vivo, in the Berkeley SCD murine model (SS). Methods Urine output was measured in metabolic cage for both wild type and SS mice (25-30 g). Bladder strips and urethra rings were dissected free and mounted in organ baths. In isolated detrusor smooth muscle (DSM), relaxant response to mirabegron and isoproterenol (1 nM-10 mu M) and contractile response to (carbachol (CCh; 1 nM-100 mu M), KCl (1 mM-300mM), CaCl2 (1 mu M-100mM), alpha,beta-methylene ATP (1, 3 and 10 mu M) and electrical field stimulation (EFS; 1-32 Hz) were measured. Phenylephrine (Phe; 10nM-100 mu M) was used to evaluate the contraction mechanism in the urethra rings. Cystometry and histomorphometry were also performed in the urinary bladder. Results SS mice present a reduced urine output and incapacity to produce typical bladder contractions and bladder emptying (ex vivo), compared to control animals. In DSM, relaxation in response to a selective beta 3-adrenergic agonist (mirabegron) and to a non-selective beta-adrenergic (isoproterenol) agonist were lower in SS mice. Additionally, carbachol, alpha,beta-methylene ATP, KCl, extracellular Ca2+ and electrical-field stimulation promoted smaller bladder contractions in SS group. Urethra contraction induced by phenylephrine was markedly reduced in SS mice. Histological analyses of SS mice bladder revealed severe structural abnormalities, such as reductions in detrusor thickness and bladder volume, and cell infiltration. Conclusions Taken together, our data demonstrate, for the first time, that SS mice display features of urinary bladder dysfunction, leading to impairment in urinary continence, which may have an important role in the pathogenesis of the enuresis and infections observed the SCD patients.
dc.description10
dc.description8
dc.description
dc.description
dc.description
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2008/57441-0]
dc.descriptionCNPq [481761/2008-0]
dc.description
dc.description
dc.description
dc.languageen
dc.publisherPUBLIC LIBRARY SCIENCE
dc.publisher
dc.publisherSAN FRANCISCO
dc.relationPLOS ONE
dc.rightsaberto
dc.sourceWOS
dc.subjectNocturnal Enuresis
dc.subjectSmooth-muscle
dc.subjectBeta-adrenoceptors
dc.subjectTract
dc.subjectContraction
dc.subjectDetrusor
dc.subjectChildren
dc.subjectOutcomes
dc.subjectTrait
dc.subjectPathophysiology
dc.titleUrinary Bladder Dysfunction In Transgenic Sickle Cell Disease Mice
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución