Capillary Electrophoresis Coupled To Contactless Conductivity Detection For The Analysis Of S-nitrosothiols Decomposition And Reactivity

dc.creatorIsmail
dc.creatorAbdulghani; d'Orlye
dc.creatorFanny; Griveau
dc.creatorSophie; Bedioui
dc.creatorFethi; Varenne
dc.creatorAnne; Fracassi da Silva
dc.creatorJose Alberto
dc.date2015-AUG
dc.date2016-06-07T13:34:21Z
dc.date2016-06-07T13:34:21Z
dc.date.accessioned2018-03-29T01:50:02Z
dc.date.available2018-03-29T01:50:02Z
dc.identifier
dc.identifierCapillary Electrophoresis Coupled To Contactless Conductivity Detection For The Analysis Of S-nitrosothiols Decomposition And Reactivity. Wiley-blackwell, v. 36, p. 1982-1988 AUG-2015.
dc.identifier0173-0835
dc.identifierWOS:000360055300026
dc.identifier10.1002/elps.201500036
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/elps.v36.16/issuetoc
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/243905
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1307603
dc.descriptionS-Nitrosothiols (RSNO) are composed of a NO group bound to the sulfhydryl group of a peptide or protein. RSNO are very important biological molecules, since they have many effects on human health. RSNO are easily naturally decomposed by metal ions, light, and heat, with different kinetics. They can furthermore undergo transnitrosation (NO moieties exchange), which is a crucial point in physiological conditions since the concentration ratios between the different nitrosothiols is a key factor in many physiopathological processes. There is therefore a great need for their quantitation. Many S-nitrosothiol detection and quantitation methods need their previous decomposition, leading thus to some limitations. We propose a direct quantitation method employing the coupling of capillary electrophoresis with a homemade capacitively coupled contactless conductivity ((CD)-D-4) detector in order to separate and quantify S-nitrosoglutathione and its decomposition products. After optimization of the method, we have studied the kinetics of decomposition using light and heat. Our results show that the decomposition by light is first order (k(obs) = (3.40 +/- 0.15) x 10(-3) s(-1)) while that using heat (at 80 degrees C) is zeroth order (k(obs,80 degrees C) = (4.34 +/- 0.14) x 10(-6) mol L-1 s(-1)). Transnitrosation reaction between S-nitrosoglutathione and cysteine was also studied, showing the possibility of separation and detection of all the products of this reaction in less than 2.5 min.
dc.description36
dc.description16
dc.description
dc.description1982
dc.description1988
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFrench Committee for the Evaluation of Academic and Scientific Cooperation with Brazil (COFECUB) [802-14]
dc.descriptionLabEx MICHEM for international mobility
dc.description
dc.description
dc.description
dc.languageen
dc.publisherWILEY-BLACKWELL
dc.publisher
dc.publisherHOBOKEN
dc.relationELECTROPHORESIS
dc.rightsfechado
dc.sourceWOS
dc.subjectNitric-oxide Release
dc.subjectMicrochip Electrophoresis
dc.subjectBiological-activity
dc.subjectNitrosoglutathione
dc.subjectThiols
dc.subjectNitrosylation
dc.subjectProtein
dc.subjectNitrate
dc.subjectFluids
dc.subjectChromatography
dc.titleCapillary Electrophoresis Coupled To Contactless Conductivity Detection For The Analysis Of S-nitrosothiols Decomposition And Reactivity
dc.typeArtículos de revistas


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