Leucine Supplementation Does Not Affect Protein Turnover And Impairs The Beneficial Effects Of Endurance Training On Glucose Homeostasis In Healthy Mice

dc.creatorCosta Junior
dc.creatorJose M.; Rosa
dc.creatorMorgana R.; Protzek
dc.creatorAndre O.; de Paula
dc.creatorFlavia M.; Ferreira
dc.creatorSandra M.; Rezende
dc.creatorLuiz F.; Vanzela
dc.creatorEmerielle C.; Zoppi
dc.creatorClaudio C.; Silveira
dc.creatorLeonardo R.; Kettelhut
dc.creatorIsis C.; Boschero
dc.creatorAntonio C.; de Oliveira
dc.creatorCamila A. M.; Carneiro
dc.creatorEverardo M.
dc.date2015-APR
dc.date2016-06-07T13:32:50Z
dc.date2016-06-07T13:32:50Z
dc.date.accessioned2018-03-29T01:48:35Z
dc.date.available2018-03-29T01:48:35Z
dc.identifier
dc.identifierLeucine Supplementation Does Not Affect Protein Turnover And Impairs The Beneficial Effects Of Endurance Training On Glucose Homeostasis In Healthy Mice. Springer Wien, v. 47, p. 745-755 APR-2015.
dc.identifier0939-4451
dc.identifierWOS:000351383900008
dc.identifier10.1007/s00726-014-1903-z
dc.identifierhttp://link.springer.com/article/10.1007%2Fs00726-014-1903-z
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/243550
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1307248
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionEndurance exercise training as well as leucine supplementation modulates glucose homeostasis and protein turnover in mammals. Here, we analyze whether leucine supplementation alters the effects of endurance exercise on these parameters in healthy mice. Mice were distributed into sedentary (C) and exercise (T) groups. The exercise group performed a 12-week swimming protocol. Half of the C and T mice, designated as the CL and TL groups, were supplemented with leucine (1.5 % dissolved in the drinking water) throughout the experiment. As well known, endurance exercise training reduced body weight and the retroperitoneal fat pad, increased soleus mass, increased VO2max, decreased muscle proteolysis, and ameliorated peripheral insulin sensitivity. Leucine supplementation had no effect on any of these parameters and worsened glucose tolerance in both CL and TL mice. In the soleus muscle of the T group, AS-160(Thr-642) (AKT substrate of 160 kDa) and AMPK(Thr-172) (AMP-Activated Protein Kinase) phosphorylation was increased by exercise in both basal and insulin-stimulated conditions, but it was reduced in TL mice with insulin stimulation compared with the T group. Akt phosphorylation was not affected by exercise but was lower in the CL group compared with the other groups. Leucine supplementation increased mTOR phosphorylation at basal conditions, whereas exercise reduced it in the presence of insulin, despite no alterations in protein synthesis. In trained groups, the total FoxO3a protein content and the mRNA for the specific isoforms E2 and E3 ligases were reduced. In conclusion, leucine supplementation did not potentiate the effects of endurance training on protein turnover, and it also reduced its positive effects on glucose homeostasis.
dc.description47
dc.description4
dc.description
dc.description745
dc.description755
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description
dc.description
dc.description
dc.languageen
dc.publisherSPRINGER WIEN
dc.publisher
dc.publisherWIEN
dc.relationAMINO ACIDS
dc.rightsfechado
dc.sourceWOS
dc.subjectSkeletal-muscle Atrophy
dc.subjectTumor-bearing Rats
dc.subjectUbiquitin Ligases
dc.subjectResistance Exercise
dc.subjectInsulin-resistance
dc.subjectPhysical-exercise
dc.subjectAmino-acids
dc.subjectRich Diet
dc.subjectAmpk
dc.subjectTranscription
dc.titleLeucine Supplementation Does Not Affect Protein Turnover And Impairs The Beneficial Effects Of Endurance Training On Glucose Homeostasis In Healthy Mice
dc.typeArtículos de revistas


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