Graphene Oxide-silver Nanocomposite As A Promising Biocidal Agent Against Methicillin-resistant Staphylococcus Aureus

dc.creatorMazarin de Moraes
dc.creatorAna Carolina; Lima
dc.creatorBruna Araujo; de Faria
dc.creatorAndreia Fonseca; Brocchi
dc.creatorMarcelo; Alves
dc.creatorOswaldo Luiz
dc.date2015
dc.date2016-06-07T13:22:53Z
dc.date2016-06-07T13:22:53Z
dc.date.accessioned2018-03-29T01:42:24Z
dc.date.available2018-03-29T01:42:24Z
dc.identifier
dc.identifierGraphene Oxide-silver Nanocomposite As A Promising Biocidal Agent Against Methicillin-resistant Staphylococcus Aureus. Dove Medical Press Ltd, v. 10, p. 6847-6861 2015.
dc.identifier1178-2013
dc.identifierWOS:000364173100001
dc.identifier10.2147/IJN.S90660
dc.identifierhttps://www.dovepress.com/graphene-oxide-silver-nanocomposite-as-a-promising-biocidal-agent-agai-peer-reviewed-article-IJN#
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/243271
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1306969
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionBackground: Methicillin-resistant Staphylococcus aureus (MRSA) has been responsible for serious hospital infections worldwide. Nanomaterials are an alternative to conventional antibiotic compounds, because bacteria are unlikely to develop microbial resistance against nanomaterials. In the past decade, graphene oxide (GO) has emerged as a material that is often used to support and stabilize silver nanoparticles (AgNPs) for the preparation of novel antibacterial nanocomposites. In this work, we report the synthesis of the graphene-oxide silver nanocomposite (GO-Ag) and its antibacterial activity against relevant microorganisms in medicine. Materials and methods: GO-Ag nanocomposite was synthesized through the reduction of silver ions (Ag+) by sodium citrate in an aqueous GO dispersion, and was extensively characterized using ultraviolet-visible absorption spectroscopy, X-ray diffraction, thermogravimetric analysis, X-ray photoelectron spectroscopy, and transmission electron microscopy. The antibacterial activity was evaluated by microdilution assays and time-kill experiments. The morphology of bacterial cells treated with GO-Ag was investigated via transmission electron microscopy. Results: AgNPs were well distributed throughout GO sheets, with an average size of 9.4 +/- 2.8 nm. The GO-Ag nanocomposite exhibited an excellent antibacterial activity against methicillin-resistant S. aureus, Acinetobacter baumannii, Enterococcus faecalis, and Escherichia coli. All (100%) MRSA cells were inactivated after 4 hours of exposure to GO-Ag sheets. In addition, no toxicity was found for either pristine GO or bare AgNPs within the tested concentration range. Transmission electronic microscopy images offered insights into how GO-Ag nanosheets interacted with bacterial cells. Conclusion: Our results indicate that the GO-Ag nanocomposite is a promising antibacterial agent against common nosocomial bacteria, particularly antibiotic-resistant MRSA. Morphological injuries on MRSA cells revealed a likely loss of viability as a result of the direct contact between bacteria and the GO-Ag sheets.
dc.description10
dc.description
dc.description
dc.description6847
dc.description6861
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionSao Paulo, Brazil)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionBrasilia, Brazil)
dc.descriptionNational Institute of Science, Technology and Innovation in Complex Functional Materials (INOMAT/INCT
dc.descriptionCampinas, Brazil)
dc.descriptionBrazilian Nanotoxicology Network (CigeNanotox)
dc.descriptionLaboratory of Synthesis of Nanostructures and Interaction with Biosystems (NanoBioss
dc.descriptionCampinas, Brazil)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description
dc.description
dc.description
dc.languageen
dc.publisherDOVE MEDICAL PRESS LTD
dc.publisher
dc.publisherALBANY
dc.relationINTERNATIONAL JOURNAL OF NANOMEDICINE
dc.rightsaberto
dc.sourceWOS
dc.subjectGram-negative Bacteria
dc.subjectAntibacterial Activity
dc.subjectEscherichia-coli
dc.subjectAntimicrobial Activity
dc.subjectNanoparticles
dc.subjectInfections
dc.subjectNanotechnology
dc.subjectNanomaterials
dc.subjectCytotoxicity
dc.subjectMechanisms
dc.titleGraphene Oxide-silver Nanocomposite As A Promising Biocidal Agent Against Methicillin-resistant Staphylococcus Aureus
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución