Adsorption Characteristics Of Cellulase And -glucosidase On Avicel, Pretreated Sugarcane Bagasse, And Lignin

dc.creatorMachado
dc.creatorDaniele Longo; Moreira Neto
dc.creatorJoao; da Cruz Pradella
dc.creatorJose Geraldo; Bonomi
dc.creatorAntonio; Rabelo
dc.creatorSarita Candida; da Costa
dc.creatorAline Carvalho
dc.date2015-SEP-OCT
dc.date2016-06-07T13:21:43Z
dc.date2016-06-07T13:21:43Z
dc.date.accessioned2018-03-29T01:41:29Z
dc.date.available2018-03-29T01:41:29Z
dc.identifier
dc.identifierAdsorption Characteristics Of Cellulase And -glucosidase On Avicel, Pretreated Sugarcane Bagasse, And Lignin. Wiley-blackwell, v. 62, p. 681-689 SEP-OCT-2015.
dc.identifier0885-4513
dc.identifierWOS:000363407100013
dc.identifier10.1002/bab.1307
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/bab.1307/abstract
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/243121
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1306819
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionAlthough adsorption is an essential step in the enzymatic hydrolysis of lignocellulosic materials, literature reports controversial results in relation to the adsorption of the cellulolitic enzymes on different biomasses/pretreatments, which makes difficult the description of this phenomenon in hydrolysis mathematical models. In this work, the adsorption of these enzymes on Avicel and sugarcane bagasse pretreated by the hydrothermal bagasse (HB) and organosolv bagasse (OB) methods was evaluated. The results have shown no significant adsorption of -glucosidase on Avicel or HB. Increasing solids concentration from 5% (w/v) to 10% (w/v) had no impact on the adsorption of cellulase on the different biomasses if stirring rates were high enough (>100 rpm for Avicel and >150rpm for HB and OB). Adsorption equilibrium time was low for Avicel (10 Min) when compared with the lignocellulosic materials (120 Min). Adsorption isotherms determined at 4 and 50 degrees C have shown that for Avicel there was a decrease in the maximum adsorption capacity (E-max) with the temperature increase, whereas for HB increasing temperature increased E-max. Also, E-max increased with the content of lignin in the material. Adsorption studies of cellulase on lignin left after enzymatic digestion of HB show lower but significant adsorption capacity (E-max = 11.92 +/- 0.76 mg/g). (C) 2014 International Union of Biochemistry and Molecular Biology, Inc.
dc.description62
dc.description5
dc.description
dc.description681
dc.description689
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2011/02743-5]
dc.description
dc.description
dc.description
dc.languageen
dc.publisherWILEY-BLACKWELL
dc.publisher
dc.publisherHOBOKEN
dc.relationBIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
dc.rightsfechado
dc.sourceWOS
dc.subjectCreeping Wild Ryegrass
dc.subjectEnzymatic-hydrolysis
dc.subjectTrichoderma-reesei
dc.subjectCorn Stover
dc.subjectMicrocrystalline Cellulose
dc.subjectLignocellulosic Biomass
dc.subjectActivity Profiles
dc.subjectSoftwood
dc.subjectDegradation
dc.subjectInhibition
dc.titleAdsorption Characteristics Of Cellulase And -glucosidase On Avicel, Pretreated Sugarcane Bagasse, And Lignin
dc.typeArtículos de revistas


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