Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The aim of this study was to evaluate the effect of germination and alcalase hydrolysis of common bean proteins on the generation of bioactive peptides with potential to reduce parameters related to the risk of developing type-2 diabetes (T2D) in vitro. Germination (25 degrees C up to 72 h) and alcalase hydrolysis (up to 4 h) produced peptides with high antioxidant capacity (1085 mu mol TE/g soluble protein, SP). After 24 h of germination, there was an increase of 44% in alpha-amylase inhibitory capacity of the peptides relative to acarbose (1 mM). Simulated gastrointestinal digestion of the non-germinated and non-hydrolyzed sample (GO-Oh) produced bioactive peptides that inhibited dipeptidyl peptidase-IV (DPP-IV) (IC50 = 1.2 mg SP/mL); however, the IC50 was not improved by either germination or alcalase hydrolysis. Insulin secretion by glucose stimulated (20 mM) INS-1E pancreatic beta-cells, increased 45% from the basal state with 2 mg SP/mL of G0-0h. Germination did not improve the stimulation of insulin. Computational modeling showed that the peptide RGPLVNPDPICPFL obtained after 48 h germination and 1 h alcalase-hydrolysis was able to inhibit DPP-IV by interacting with its S1, S2, and S3 pockets of the active site. Germination and alcalase hydrolysis can be applied to improve some markers related to the management of T2D. Furthermore, simulated gastrointestinal digestion of common bean proteins without any treatment produced bioactive peptides with the ability to inhibit DPP-IV, resulting in increased insulin released from pancreatic cells in vitro. (C) 2015 Elsevier Ltd. All rights reserved.76 150159Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Hatch grant, University of IllinoisNational Science Foundation (NSF) Mentoring in New Biology Fellowship [1041233]Multicultural Advocates in Nutrition Needs & Agriculture (MANNA) scholarship - USDA [2011-38413-30107]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)