Sequential Optimization Approach For Prebiotic Galactooligosaccharides Synthesis By Pseudozyma Tsukubaensis And Pichia Kluyveri

dc.creatorCavalcante Fai
dc.creatorAna Elizabeth; Resende Simiqueli
dc.creatorAna Paula; Ghiselli
dc.creatorGislaine; Pastore
dc.creatorGlaucia Maria
dc.date2015-OCT
dc.date2016-06-07T13:19:32Z
dc.date2016-06-07T13:19:32Z
dc.date.accessioned2018-03-29T01:39:43Z
dc.date.available2018-03-29T01:39:43Z
dc.identifier
dc.identifierSequential Optimization Approach For Prebiotic Galactooligosaccharides Synthesis By Pseudozyma Tsukubaensis And Pichia Kluyveri. Elsevier Science Bv, v. 63, p. 1214-1219 OCT-2015.
dc.identifier0023-6438
dc.identifierWOS:000356545500059
dc.identifier10.1016/j.lwt.2015.04.064
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0023643815003461
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/242711
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1306409
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionSynthesis of the prebiotic galactooligosaccharides from lactose fermentation by Pseudozyma tsukubaensis and Pichia kluyveri was investigated. In this study, a sequential strategy of two experimental designs was used to optimize galactooligosaccharides production. First, Plackett-Burman screening design was applied to evaluate the effects of lactose, yeast extract, urea concentration, inoculum concentration, pH and agitation rate on the response of galactooligosaccharides yield synthesized by both yeasts. The preliminary Plackett-Burman design allowed the identification of the pH and urea concentration as statistically significant variables in the range studied. In order to find the optimal conditions for galactooligosaccharides synthesis, a central composite rotational design was employed. The independent variables were lactose, yeast extract and urea concentrations, and the response taken into account was galactooligosaccharides yield produced by P. tsukubaensis. The optimized condition for this prebiotic synthesis was: lactose, yeast extract and urea at concentrations of 26.0, 0.8 and 1.8 g/100 mL respectively. Under the optimized and validated conditions, the transgalactosylation activity of P. tsukubaensis resulted in GOS yield of 28.35 GOS (g)/100 g of lactose at 24 h, with a maximum GOS production of 73.71 g/L, and approximately 50 g/100 g of lactose hydrolysis. (C) 2015 Elsevier Ltd. All rights reserved.
dc.description63
dc.description2
dc.description
dc.description1214
dc.description1219
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionUniversidade Estadual de Campinas (Unicamp)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description
dc.description
dc.description
dc.languageen
dc.publisherELSEVIER SCIENCE BV
dc.publisher
dc.publisherAMSTERDAM
dc.relationLWT-FOOD SCIENCE AND TECHNOLOGY
dc.rightsembargo
dc.sourceWOS
dc.subjectGalacto-oligosaccharide Production
dc.subjectBeta-galactosidase
dc.subjectEnzymatic-synthesis
dc.subjectLactose Hydrolysis
dc.subjectLactobacillus
dc.subjectSingularis
dc.subjectDesign
dc.subjectCells
dc.subjectMilk
dc.titleSequential Optimization Approach For Prebiotic Galactooligosaccharides Synthesis By Pseudozyma Tsukubaensis And Pichia Kluyveri
dc.typeArtículos de revistas


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