Mmp-9/reck Imbalance: A Mechanism Associated With High-grade Cervical Lesions And Genital Infection By Human Papillomavirus And Chlamydia Trachomatis

dc.creatorDiscacciati
dc.creatorMichelle G.; Gimenes
dc.creatorFabrcia; Pennacchi
dc.creatorPaula C.; Faiao-Flores
dc.creatorFernanda; Zeferino
dc.creatorLuiz C.; Derchain
dc.creatorSophie M.; Teixeira
dc.creatorJulio C.; Costa
dc.creatorMaria C.; Zonta
dc.creatorMarco; Termini
dc.creatorLara; Boccardo
dc.creatorEnrique; Longatto-Filho
dc.creatorAdhemar; Consolaro
dc.creatorMarcia E. L.; Villa
dc.creatorLuisa L.; Maria-Engler
dc.creatorSilvya S.
dc.date2015-OCT
dc.date2016-06-07T13:19:29Z
dc.date2016-06-07T13:19:29Z
dc.date.accessioned2018-03-29T01:39:41Z
dc.date.available2018-03-29T01:39:41Z
dc.identifier
dc.identifierMmp-9/reck Imbalance: A Mechanism Associated With High-grade Cervical Lesions And Genital Infection By Human Papillomavirus And Chlamydia Trachomatis. Amer Assoc Cancer Research, v. 24, p. 1539-1547 OCT-2015.
dc.identifier1055-9965
dc.identifierWOS:000363401800012
dc.identifier10.1158/1055-9965.EPI-15-0420
dc.identifierhttp://cebp.aacrjournals.org/content/24/10/1539
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/242699
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1306397
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionBackground: Matrix metalloproteinases (MMP) are important enzymes in the tumor microenvironment associated with progression of cervical intraepithelial neoplasia (CIN) toward squamous cell carcinoma (SCC) of the cervix. However, the role of MMPs in the inflammatory process associated with Chlamydia trachomatis infection concomitant with the carcinogenic process driven by HPV has not yet been addressed. In the present study, we analyzed the state of the MMP-9-RECK axis in cervical carcinogenesis. Methods: The levels of MMP-9 and RECK expression were analyzed by immunocytochemistry in liquid-based cytology samples from 136 women with high-grade cervical lesions (CIN2/CIN3) and cervical SCC diagnosed by LLETZ, and in 196 women without cervical neoplasia or CIN1. Real-time qPCR was performed to analyze expression of MMP-9 and RECK in 15 cervical samples. The presence of HPV-DNA and other genital pathogens was evaluated by PCR. Results: We found a higher expression of MMP-9 [OR, 4.2; 95% confidence interval (CI), 2.2-7.8] and lower expression of RECK (OR, 0.4; 95% CI, 0.2-0.7) in women with CIN2/CIN3/SCC when compared with women from the control group (no neoplasia/CIN1). A statistically significant association was also found between MMP-9/RECK imbalance and infection by alpha-9 HPV and C. trachomatis. The prevalence of C. trachomatis infection was significantly higher in women with high-grade cervical disease (OR, 3.7; 95% CI, 1.3-11.3). Conclusions: MMP-9/RECK imbalance in cervical smears is significantly associated with high-grade cervical diseases and infection by alpha-9 HPV and C. trachomatis. Impact: MMP-9/RECK imbalance during cervical inflammation induced by C. trachomatis might play a role in HPV-mediated cervical carcinogenesis. (C) 2015 AACR.
dc.description24
dc.description10
dc.description
dc.description1539
dc.description1547
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2008/03232-1, 2012/09746-2]
dc.description
dc.description
dc.description
dc.languageen
dc.publisherAMER ASSOC CANCER RESEARCH
dc.publisher
dc.publisherPHILADELPHIA
dc.relationCANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
dc.rightsfechado
dc.sourceWOS
dc.subjectMatrix Metalloproteinases
dc.subjectIntraepithelial Neoplasia
dc.subjectBacterial Vaginosis
dc.subjectPatricia Trial
dc.subjectHpv Infection
dc.subjectKazal Motifs
dc.subjectIn-vitro
dc.subjectCancer
dc.subjectWomen
dc.subjectRisk
dc.titleMmp-9/reck Imbalance: A Mechanism Associated With High-grade Cervical Lesions And Genital Infection By Human Papillomavirus And Chlamydia Trachomatis
dc.typeArtículos de revistas


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