Differentiation Of C57/bl6 Mice Bone Marrow Mononuclear Cells Into Early Endothelial Progenitors Cells In Different Culture Conditions

dc.creatorCarneiro
dc.creatorGiane D.; Godoy
dc.creatorJuliana A. P.; Werneck
dc.creatorClaudio C.; Vicente
dc.creatorCristina P.
dc.date2015-OCT
dc.date2016-06-07T13:19:23Z
dc.date2016-06-07T13:19:23Z
dc.date.accessioned2018-03-29T01:39:34Z
dc.date.available2018-03-29T01:39:34Z
dc.identifier
dc.identifierDifferentiation Of C57/bl6 Mice Bone Marrow Mononuclear Cells Into Early Endothelial Progenitors Cells In Different Culture Conditions. Wiley-blackwell, v. 39, p. 1138-1150 OCT-2015.
dc.identifier1065-6995
dc.identifierWOS:000362790900007
dc.identifier10.1002/cbin.10487
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/cbin.10487/epdf
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/242672
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1306370
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionEndothelial progenitor cells (EPCs) can be isolated from bone marrow and characterized by the expression of cellular markers such as CD34, CD133, VEGFR2, CD31, and VE-Cadherin, by the uptake of acetylated low-density lipoprotein and by in vitro tube formation in tridimensional matrices. These cells are able to differentiate into mature endothelial cells and participate in the re-endothelization of damaged vessels. In this work, we tested different cultured media that can promote the proliferation and differentiation of mononuclear cells (MNCs) into early EPCs, with defined concentrations of growth factors and serum in order to establish a composition that may ensure us the reproducibility of our cultures. MNCs from mice bone marrow were cultivated using selective culture media containing DMEM or M199 supplemented with 10% FBS, VEGF, bFGF, and IGF, for 3, 7, and 14 days. Differentiation into early EPCs was analyzed using immunohistochemistry, FACS and western blotting and by functional parameters as uptake of ac-LDL, and formation of vessel-like structures. The cells cultivated with medium DMEM-M1 (DMEM plus VEGF, bFGF and IGF) expressed CD34, CD133, CD31, VEGFR2, and VE-Cadherin at all culture time-points with increased expression of these markers after 7 days. Only EPCs cultured for 30 days were able to form vessel-like structure. The uptake of ac-LDL was observed after 3, 7, 14, and 30 days, confirming the differentiation of mononuclear cells into early EPCs. DMEM-M1 was able to sustain MNCs proliferation and differentiation, increasing the expression of the characteristic EPC markers, allowing the expansion of early EPCs in culture in a similar way to that observed in commercial available media.
dc.description39
dc.description10
dc.description
dc.description1138
dc.description1150
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2012/23640-2, 2010/19916-7]
dc.descriptionCNPq [308520/2010-6, 307784/2013-4]
dc.description
dc.description
dc.description
dc.languageen
dc.publisherWILEY-BLACKWELL
dc.publisher
dc.publisherHOBOKEN
dc.relationCELL BIOLOGY INTERNATIONAL
dc.rightsfechado
dc.sourceWOS
dc.subjectUmbilical-cord Blood
dc.subjectIn-vitro
dc.subjectPeripheral-blood
dc.subjectPar-1 Activation
dc.subjectShear-stress
dc.subjectAngiogenesis
dc.subjectOutgrowth
dc.subjectIdentification
dc.subjectDisease
dc.subjectNeovascularization
dc.titleDifferentiation Of C57/bl6 Mice Bone Marrow Mononuclear Cells Into Early Endothelial Progenitors Cells In Different Culture Conditions
dc.typeArtículos de revistas


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