Unmanipulated Native Fat Exposed To High-energy Diet, But Not Autologous Grafted Fat By Itself, May Lead To Overexpression Of Ki67 And Pai-1

dc.creatorClaro
dc.creatorFrancisco
dc.creatorJr.; Morari
dc.creatorJoseane; Moreira
dc.creatorLuciana R.; Sarian
dc.creatorLuis O. Z.; Pinto
dc.creatorGlauce A.; Velloso
dc.creatorLicio A.; Pinto-Neto
dc.creatorAarao M.
dc.date2015-Jun
dc.date2016-06-07T13:18:46Z
dc.date2016-06-07T13:18:46Z
dc.date.accessioned2018-03-29T01:39:05Z
dc.date.available2018-03-29T01:39:05Z
dc.identifier
dc.identifierUnmanipulated Native Fat Exposed To High-energy Diet, But Not Autologous Grafted Fat By Itself, May Lead To Overexpression Of Ki67 And Pai-1. Springer International Publishing Ag, v. 4, p. Jun-2015.
dc.identifier2193-1801
dc.identifierWOS:000359261200002
dc.identifier10.1186/s40064-015-1061-0
dc.identifierhttp://springerplus.springeropen.com/articles/10.1186/s40064-015-1061-0
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/242573
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1306271
dc.descriptionBackground: Although its unclear oncological risk, which led to more than 20 years of prohibition of its use, fat grafting to the breast is widely used nowadays even for aesthetic purposes. Thus, we proposed an experimental model in rats to analyze the inflammatory activity, cellular proliferation and levels of Plasminogen Activator Inhibitor (PAI-1) in grafted fat, and in native fat exposed to high-energy diet in order to study the oncological potential of fat tissue. Methods: Samples of grafted fat of rats on regular-energy diet were compared with paired samples of native fat from the same rat on regular-energy diet and on high-energy diet in a different time. Analysis involved microscopic comparisons using hematoxylin-eosin staining, immunohistochemistry with anti-CD68-labelled macrophages, and gene expression of Ki-67 and PAI-1. Results: Hematoxylin-eosin staining analyses did not find any atypical cellular infiltration or unusual tissue types in the samples of grafted fat. The inflammatory status, assessed through immunohistochemical identification of CD68-labelled macrophages, was similar among samples of native fat and grafted fat of rat on regular-energy diet and of native fat of rats on high-energy diet. Real-time PCR revealed that high-energy diet, but not fat grafting, leads to proliferative status on adipose tissue (overexpression of ki-67, p = 0.046) and raised its PAI-1 levels, p < 0.001. Conclusion: While the native adipose tissue overexpressed PAI-1 and KI67 when exposed to high-energy diet, the grafted fat by itself was unable to induce cellular proliferation, chronic inflammatory activity and/or elevation of PAI-1 levels.
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dc.languageen
dc.publisherSPRINGER INTERNATIONAL PUBLISHING AG
dc.publisher
dc.publisherCHAM
dc.relationSPRINGERPLUS
dc.rightsaberto
dc.sourceWOS
dc.subjectPlasminogen-activator Inhibitor-1
dc.subjectBreast-cancer Patients
dc.subjectTumor-cell Migration
dc.subjectMammary-gland
dc.subjectAdipose-tissue
dc.subjectMacrophage Accumulation
dc.subjectOmental Transposition
dc.subjectSubcutaneous Fat
dc.subjectGreater Omentum
dc.subjectParacrine Loop
dc.titleUnmanipulated Native Fat Exposed To High-energy Diet, But Not Autologous Grafted Fat By Itself, May Lead To Overexpression Of Ki67 And Pai-1
dc.typeArtículos de revistas


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