Violacein Treatment Modulates Acute And Chronic Inflammation Through The Suppression Of Cytokine Production And Induction Of Regulatory T Cells

dc.creatorVerinaud
dc.creatorLiana; Pinto Lopes
dc.creatorStefanie Costa; Naranjo Prado
dc.creatorIsabel Cristina; Zanucoli
dc.creatorFabio; da Costa
dc.creatorThiago Alves; Di Gangi
dc.creatorRosaria; Issayama
dc.creatorLuidy Kazuo; Carvalho
dc.creatorAna Carolina; Bonfanti
dc.creatorAmanda Pires; Niederauer
dc.creatorGuilherme Francio; Duran
dc.creatorNelson; Maranhao Costa
dc.creatorFabio Trindade; Rodrigues Oliveira
dc.creatorAlexandre Leite; da Cruz Hoefling
dc.creatorMaria Alice; Stach Machado
dc.creatorDagmar Ruth; Thome
dc.creatorRodolfo
dc.date2015-MAY
dc.date2016-06-07T13:18:11Z
dc.date2016-06-07T13:18:11Z
dc.date.accessioned2018-03-29T01:38:37Z
dc.date.available2018-03-29T01:38:37Z
dc.identifier
dc.identifierViolacein Treatment Modulates Acute And Chronic Inflammation Through The Suppression Of Cytokine Production And Induction Of Regulatory T Cells. Public Library Science, v. 10, p. MAY-2015.
dc.identifier1932-6203
dc.identifierWOS:000353943000065
dc.identifier10.1371/journal.pone.0125409
dc.identifierhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0125409
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/242461
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1306159
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionInflammation is a necessary process to control infection. However, exacerbated inflammation, acute or chronic, promotes deleterious effects in the organism. Violacein (viola), a quorum sensing metabolite from the Gram-negative bacterium Chromobacterium violaceum, has been shown to protect mice from malaria and to have beneficial effects on tumors. However, it is not known whether this drug possesses anti-inflammatory activity. In this study, we investigated whether viola administration is able to reduce acute and chronic autoimmune inflammation. For that purpose, C57BL/6 mice were intraperitoneally injected with 1 mu g of LPS and were treated with viola (3.5mg/kg) via i.p. at the same time-point. Three hours later, the levels of inflammatory cytokines in the sera and phenotypical characterization of leukocytes were determined. Mice treated with viola presented a significant reduction in the production of inflammatory cytokines compared with untreated mice. Interestingly, although viola is a compound derived from bacteria, it did not induce inflammation upon administration to naive mice. To test whether viola would protect mice from an autoimmune inflammation, Experimental Autoimmune Encephalomyelitis (EAE)-inflicted mice were given viola i.p. at disease onset, at the 10th day from immunization. Viola-treated mice developed mild EAE disease in contrast with placebo-treated mice. The frequencies of dendritic cells and macrophages were unaltered in EAE mice treated with viola. However, the sole administration of viola augmented the levels of splenic regulatory T cells (CD4+ Foxp3+). We also found that adoptive transfer of viola-elicited regulatory T cells significantly reduced EAE. Our study shows, for the first time, that violacein is able to modulate acute and chronic inflammation. Amelioration relied in suppression of cytokine production (in acute inflammation) and stimulation of regulatory T cells (in chronic inflammation). New studies must be conducted in order to assess the possible use of viola in therapeutic approaches in human autoimmune diseases.
dc.description10
dc.description5
dc.description
dc.description
dc.description
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [2011/17965-3]
dc.descriptionCNPq [471066/2012-5]
dc.descriptionFAPESP [2014/02631-0, 2011/23664-6, 2012/01892-0]
dc.description
dc.description
dc.description
dc.languageen
dc.publisherPUBLIC LIBRARY SCIENCE
dc.publisher
dc.publisherSAN FRANCISCO
dc.relationPLOS ONE
dc.rightsaberto
dc.sourceWOS
dc.subjectExperimental Autoimmune Encephalomyelitis
dc.subjectSystemic-lupus-erythematosus
dc.subjectCollagen-induced Arthritis
dc.subjectDendritic Cells
dc.subjectChromobacterium-violaceum
dc.subjectImmune-responses
dc.subjectIn-vitro
dc.subjectTolerance
dc.subjectMice
dc.subjectChloroquine
dc.titleViolacein Treatment Modulates Acute And Chronic Inflammation Through The Suppression Of Cytokine Production And Induction Of Regulatory T Cells
dc.typeArtículos de revistas


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