Comparative Study Of The Bioconversion Process Using R-(+)- And S-(-)-limonene As Substrates For Fusarium Oxysporum 152b

dc.creatorMolina
dc.creatorGustavo; Bution
dc.creatorMurillo L.; Bicas
dc.creatorJuliano L.; Dolder
dc.creatorMary Anne Heidi; Pastore
dc.creatorGlaucia M.
dc.date2015-MAY
dc.date2016-06-07T13:17:47Z
dc.date2016-06-07T13:17:47Z
dc.date.accessioned2018-03-29T01:38:20Z
dc.date.available2018-03-29T01:38:20Z
dc.identifier
dc.identifierComparative Study Of The Bioconversion Process Using R-(+)- And S-(-)-limonene As Substrates For Fusarium Oxysporum 152b. Elsevier Sci Ltd, v. 174, p. 606-613 MAY-2015.
dc.identifier0308-8146
dc.identifierWOS:000348088000083
dc.identifier10.1016/j.foodchem.2014.11.059
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0308814614017889
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/242409
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1306107
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThis study compared the bioconversion process of S-(-)-limonene into limonene-1,2-diol with the already established biotransformation of R-(+)-limonene into alpha-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B. The bioconversion of the S-(-)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. When submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limonene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment, indicating a complete loss of cell viability. Furthermore, the results showed that this process can be characterised as an aerobic system that was catalysed by limonene-1,2-epoxide hydrolase, had an intracellular nature and was cofactor-dependent because the final product was not detected by an anaerobic process. Finally, this is the first report to characterise the bioconversion of R-(+)- and S-(-)-limonene by cellular detoxification using ultra-structural analysis. (C) 2014 Elsevier Ltd. All rights reserved.
dc.description174
dc.description
dc.description
dc.description606
dc.description613
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2011/50687-7]
dc.descriptionCNPq [481487/2011-5, 473981/2012-2]
dc.description
dc.description
dc.description
dc.languageen
dc.publisherELSEVIER SCI LTD
dc.publisher
dc.publisherOXFORD
dc.relationFOOD CHEMISTRY
dc.rightsembargo
dc.sourceWOS
dc.subjectSolid-phase Microextraction
dc.subjectAlpha-pinene Oxide
dc.subjectPermeabilized Cells
dc.subjectMass-transfer
dc.subjectBiotransformation
dc.subjectLimonene
dc.subjectPseudomonas
dc.subjectR-(+)-alpha-terpineol
dc.subjectTerpenes
dc.subjectFungi
dc.titleComparative Study Of The Bioconversion Process Using R-(+)- And S-(-)-limonene As Substrates For Fusarium Oxysporum 152b
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución