In Vivo Determination Of The Volatile Metabolites Of Saprotroph Fungi By Comprehensive Two-dimensional Gas Chromatography

dc.creatorde Lima
dc.creatorPaula Feliciano; Furlan
dc.creatorMayra Fontes; Ribeiro
dc.creatorFabiana Alves de Lima; Pascholati
dc.creatorSergio Florentino; Augusto
dc.creatorFabio
dc.date2015-JUN
dc.date2016-06-07T13:16:07Z
dc.date2016-06-07T13:16:07Z
dc.date.accessioned2018-03-29T01:36:43Z
dc.date.available2018-03-29T01:36:43Z
dc.identifier
dc.identifierIn Vivo Determination Of The Volatile Metabolites Of Saprotroph Fungi By Comprehensive Two-dimensional Gas Chromatography. Wiley-v C H Verlag Gmbh, v. 38, p. 1924-1932 JUN-2015.
dc.identifier1615-9306
dc.identifierWOS:000355836300014
dc.identifier10.1002/jssc.201401404
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/jssc.201401404/abstract
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/242003
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1305701
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionIn this work, we discuss the use of multiway principal component analysis combined with comprehensive two-dimensional gas chromatography to study the volatile metabolites of the saprophytic fungus Memnoniella sp. isolated in vivo by headspace solid-phase microextraction. This fungus has been identified as having the ability to induce plant resistance against pathogens, possibly through its volatile metabolites. Adequate culture media were inoculated, and its headspace was then sampled with a solid-phase microextraction fiber and chromatographed every 24 h over seven days. The raw chromatogram processing using multiway principal component analysis allowed the determination of the inoculation period, during which the concentration of volatile metabolites was maximized, as well as the discrimination of the appropriate peaks from the complex culture media background. Several volatile metabolites not previously described in the literature on biocontrol fungi were observed, as well as sesquiterpenes and aliphatic alcohols. These results stress that, due to the complexity of multidimensional chromatographic data, multivariate tools might be mandatory even for apparently trivial tasks, such as the determination of the temporal profile of metabolite production and extinction. However, when compared with conventional gas chromatography, the complex data processing yields a considerable improvement in the information obtained from the samples.
dc.description38
dc.description11
dc.description
dc.description1924
dc.description1932
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description
dc.description
dc.description
dc.languageen
dc.publisherWILEY-V C H VERLAG GMBH
dc.publisher
dc.publisherWEINHEIM
dc.relationJOURNAL OF SEPARATION SCIENCE
dc.rightsfechado
dc.sourceWOS
dc.subjectSolid-phase Microextraction
dc.subjectMultivariate Data-analysis
dc.subjectFlight Mass-spectrometry
dc.subjectGc-ms
dc.subjectFunctional Genomics
dc.subjectPlant Metabolomics
dc.subjectBiological-control
dc.subjectOrganic-compounds
dc.subjectIdentification
dc.subjectChemometrics
dc.titleIn Vivo Determination Of The Volatile Metabolites Of Saprotroph Fungi By Comprehensive Two-dimensional Gas Chromatography
dc.typeArtículos de revistas


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