Combined Nitric Oxide-releasing Poly(vinyl Alcohol) Film/f127 Hydrogel For Accelerating Wound Healing

dc.creatorSchanuel
dc.creatorFernanda Seabra; Raggio Santos
dc.creatorKaren Slis; Monte-Alto-Costa
dc.creatorAndrea; de Oliveira
dc.creatorMarcelo G.
dc.date2015-JUN
dc.date2016-06-07T13:15:56Z
dc.date2016-06-07T13:15:56Z
dc.date.accessioned2018-03-29T01:36:34Z
dc.date.available2018-03-29T01:36:34Z
dc.identifier
dc.identifierCombined Nitric Oxide-releasing Poly(vinyl Alcohol) Film/f127 Hydrogel For Accelerating Wound Healing. Elsevier Science Bv, v. 130, p. 182-191 JUN-2015.
dc.identifier0927-7765
dc.identifierWOS:000356735300024
dc.identifier10.1016/j.colsurfb.2015.04.007
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0927776515002131
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/241964
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1305662
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionNitric oxide (NO) releasing biomaterials represent a potential strategy for use as active wound dressings capable of accelerating wound healing. Topical NO-releasing poly(vinyl alcohol) (PVA) films and Pluronic F127 hydrogels (F127) have already exhibited effective skin vasodilation and wound healing actions. In this study, we functionalized PVA films with SNO groups via esterification with a mixture of mercaptosucinic acid (MSA) and thiolactic acid (TLA) followed by S-nitrosation of the -SH moieties. These films were combined with an underlying layer of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), i.e., PEO-PPO-PEO (Pluronic F127) hydrogel and used for the topical treatment of skin lesions in an animal model. The mixed esterification of PVA with MSA and TLA led to chemically crosslinked PVA-SNO films with a high swelling capacity capable of spontaneously releasing NO. Real time NO-release measurements revealed that the hydrogel layer reduces the initial NO burst from the PVA-SNO films. We demonstrate that the combination of PVA-SNO films with F127 hydrogel accelerates wound contraction, decreases wound gap and cellular density and accelerates the inflammatory phase of the lesion. These results were reflected in an increase in myofibroblastic differentiation and collagen type III expression in the cicatricial tissue. Therefore, PVA-SNO films combined with F127 hydrogel may represent a new approach for active wound dressings capable of accelerating wound healing. (C) 2015 Elsevier B.V. All rights reserved.
dc.description130
dc.description
dc.description
dc.description182
dc.description191
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCNPq [131917/2013-7]
dc.descriptionFAPESP [2013/21936-4]
dc.descriptionCNPq [309390/2011-7, 302918/2012-7]
dc.descriptionFAPESP [2008/57560-0]
dc.description
dc.description
dc.description
dc.languageen
dc.publisherELSEVIER SCIENCE BV
dc.publisher
dc.publisherAMSTERDAM
dc.relationCOLLOIDS AND SURFACES B-BIOINTERFACES
dc.rightsembargo
dc.sourceWOS
dc.subjectS-nitrosothiols
dc.subjectRepair
dc.subjectMice
dc.subjectInhibition
dc.subjectDonors
dc.subjectKeratinocytes
dc.subjectVasodilation
dc.subjectCollagen
dc.subjectFilms
dc.subjectSkin
dc.titleCombined Nitric Oxide-releasing Poly(vinyl Alcohol) Film/f127 Hydrogel For Accelerating Wound Healing
dc.typeArtículos de revistas


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