Biomarker Evaluation In Fish After Prolonged Exposure To Nano-tio2: Influence Of Illumination Conditions And Crystal Phase

dc.creatorClemente
dc.creatorZ.; Castro
dc.creatorV. L.; Feitosa
dc.creatorL. O.; Lima
dc.creatorR.; Jonsson
dc.creatorC. M.; Maia
dc.creatorA. H. N.; Fraceto
dc.creatorL. F.
dc.date2015-JUL
dc.date2016-06-07T13:15:18Z
dc.date2016-06-07T13:15:18Z
dc.date.accessioned2018-03-29T01:36:02Z
dc.date.available2018-03-29T01:36:02Z
dc.identifier
dc.identifierBiomarker Evaluation In Fish After Prolonged Exposure To Nano-tio2: Influence Of Illumination Conditions And Crystal Phase. Amer Scientific Publishers, v. 15, p. 5424-5433 JUL-2015.
dc.identifier1533-4880
dc.identifierWOS:000348489200118
dc.identifier10.1166/jnn.2015.10021
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/26373153
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/241843
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1305541
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionIn this study, we evaluated the effects of prolonged exposure to two different nano-TiO2 crystal phases under different illumination conditions. Fish (Piaractus mesopotamicus) were exposed for 21 days to 100 mg/L of nano-TiO2 anatase and a mixture of anatase:rutile (80%:20%) under visible light and UV light (UVA and B, 22.47 J/cm(2)/h). The following oxidative stress biomarkers were monitored: concentrations of lipid hydroperoxide (LPO), carbonylated proteins (PCO), and specific activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Other biomarkers as well as specific activities of acid phosphatase (AP), Na+, K+-ATPase and metallothionein levels (MT) were also evaluated. Moreover, micronucleus and comet assays were performed to assess genotoxicity. Our results showed low toxicity of nano-TiO2 to fish and lack of titanium accumulation in muscle tissue. However, it was observed the occurrence of sublethal effects that were influenced by nano-TiO2 crystal phase and illumination condition. Pure anatase caused more oxidative damage without co-exposure to UV, while the mixture anatase:rutile caused more sublethal effects when exposure occurred under UV. These findings show that the specific activity of CAT, GST, PCO levels and comet assay are useful as biomarkers of prolonged exposure to nano-TiO2. Overall, our study substantiates the development and implementation of nanoecotoxicological protocols.
dc.description15
dc.description7
dc.description
dc.description5424
dc.description5433
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [Proc. 2010/06226-2]
dc.description
dc.description
dc.description
dc.languageen
dc.publisherAMER SCIENTIFIC PUBLISHERS
dc.publisher
dc.publisherVALENCIA
dc.relationJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
dc.rightsfechado
dc.sourceWOS
dc.subjectTitanium-dioxide Nanoparticles
dc.subjectTrout Oncorhynchus-mykiss
dc.subjectZebrafish Danio-rerio
dc.subjectOxidative Dna-damage
dc.subjectTio2 Nanoparticles
dc.subjectRainbow-trout
dc.subjectIn-vitro
dc.subjectAquatic Organisms
dc.subjectDaphnia-magna
dc.subjectStress
dc.titleBiomarker Evaluation In Fish After Prolonged Exposure To Nano-tio2: Influence Of Illumination Conditions And Crystal Phase
dc.typeArtículos de revistas


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