A Leucine-rich Diet Modulates The Tumor-induced Down-regulation Of The Mapk/erk And Pi3k/akt/mtor Signaling Pathways And Maintains The Expression Of The Ubiquitin-proteasome Pathway In The Placental Tissue Of Nmri Mice

dc.creatorViana
dc.creatorLais Rosa; Cintra Gomes-Marcondes
dc.creatorMaria Cristina
dc.date2015-FEB
dc.date2016-06-07T13:13:32Z
dc.date2016-06-07T13:13:32Z
dc.date.accessioned2018-03-29T01:34:34Z
dc.date.available2018-03-29T01:34:34Z
dc.identifier
dc.identifierA Leucine-rich Diet Modulates The Tumor-induced Down-regulation Of The Mapk/erk And Pi3k/akt/mtor Signaling Pathways And Maintains The Expression Of The Ubiquitin-proteasome Pathway In The Placental Tissue Of Nmri Mice. Soc Study Reproduction, v. 92, p. FEB-2015.
dc.identifier0006-3363
dc.identifierWOS:000350807000006
dc.identifier10.1095/biolreprod.114.123307
dc.identifierhttp://www.biolreprod.org/content/92/2/49
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/241500
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1305198
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionPlacental tissue injury is concomitant with tumor development. We investigated tumor-driven placental damage by tracing certain steps of the protein synthesis and degradation pathways under leucine-rich diet supplementation in MAC16 tumor-bearing mice. Cell signaling and ubiquitin-proteasome pathways were assessed in the placental tissues of pregnant mice, which were distributed into three groups on a control diet (pregnant control, tumor-bearing pregnant, and pregnant injected with MAC-ascitic fluid) and three other groups on a leucine-rich diet (pregnant, tumor-bearing pregnant, and pregnant injected with MAC-ascitic fluid). MAC tumor growth down-regulated the cell-signaling pathways of the placental tissue and decreased the levels of IRS-1, Akt/PKB, Erk/MAPK, mTOR, p70S6K, STAT3, and STAT6 phosphorylated proteins, as assessed by the multiplex Millipore Luminex assay. Leucine supplementation maintained the levels of these proteins within the established cell-signaling pathways. In the tumor-bearing group (MAC) only, the placental tissue showed increased PC5 mRNA expression, as assessed by quantitative RT-PCR, decreased 19S and 20S protein expression, as assessed by Western blot analysis, and decreased placental tyrosine levels, likely reflecting upregulation of the ubiquitin-proteasome pathway. Similar effects were found in the pregnant injected with MAC-ascitic fluid group, confirming that the effects of the tumor were mimicked by MAC-ascitic fluid injection. Although tumor progression occurred, the degradation pathway-related protein levels were modulated under leucine-supplementation conditions. In conclusion, tumor evolution reduced the protein expression of the cell-signaling pathway associated with elevated protein degradation, thereby jeopardizing placental activity. Under the leucine-rich diet, the impact of cancer on placental function could be minimized by improving the cell-signaling activity and reducing the proteolytic process.
dc.description92
dc.description2
dc.description
dc.description
dc.description
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2009/11982-3, 2010/00209-9, 2012/06955-0]
dc.descriptionCNPq [304604/2010-0, 302863/2013-3]
dc.description
dc.description
dc.description
dc.languageen
dc.publisherSOC STUDY REPRODUCTION
dc.publisher
dc.publisherMADISON
dc.relationBIOLOGY OF REPRODUCTION
dc.rightsfechado
dc.sourceWOS
dc.subjectStimulates Protein-synthesis
dc.subjectSkeletal-muscle
dc.subjectCancer Cachexia
dc.subjectBearing Rats
dc.subjectBody-composition
dc.subjectNeonatal Pigs
dc.subjectBreast-cancer
dc.subjectPregnant Rats
dc.subjectAmino-acids
dc.subjectDependent Proteolysis
dc.titleA Leucine-rich Diet Modulates The Tumor-induced Down-regulation Of The Mapk/erk And Pi3k/akt/mtor Signaling Pathways And Maintains The Expression Of The Ubiquitin-proteasome Pathway In The Placental Tissue Of Nmri Mice
dc.typeArtículos de revistas


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