Artículos de revistas
Pbmcs Express A Transcriptome Signature Predictor Of Oxygen Uptake Responsiveness To Endurance Exercise Training In Men
Registro en:
Pbmcs Express A Transcriptome Signature Predictor Of Oxygen Uptake Responsiveness To Endurance Exercise Training In Men. Amer Physiological Soc, v. 47, p. 13-23 FEB-2015.
1094-8341
WOS:000350804900001
10.1152/physiolgenomics.00072.2014
Autor
Dias
Rodrigo Goncalves; Moreira Silva
Michelle Sabrina; Duarte
Nubia Esteban; Bolani
Wladimir; Alves
Cleber Rene; Lemos Junior
Jose Ribeiro; da Silva
Jeferson Luis; de Oliveira
Patricia Alves; Alves
Guilherme Barreto; de Oliveira
Edilamar Menezes; Rocha
Cristiane Souza; Carneiro Marsiglia
Julia Daher; Negrao
Carlos Eduardo; Krieger
Eduardo Moacyr; Krieger
Jose Eduardo; Pereira
Alexandre Costa
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Peripheral blood cells are an accessible environment in which to visualize exercise-induced alterations in global gene expression patterns. We aimed to identify a peripheral blood mononuclear cell (PBMC) signature represented by alterations in gene expression, in response to a standardized endurance exercise training protocol. In addition, we searched for molecular classifiers of the variability in oxygen uptake ((V) over dotO(2)). Healthy untrained policemen recruits (n = 13, 25 +/- 3 yr) were selected. Peak (V) over dotO(2) (measured by cardiopulmonary exercise testing) and total RNA from PBMCs were obtained before and after 18 wk of running endurance training (3 times/wk, 60 min). Total RNA was used for whole genome expression analysis using Affymetrix GeneChip Human Gene 1.0 ST. Data were normalized by the robust multiarray average algorithm. Principal component analysis was used to perform correlations between baseline gene expression and (V) over dotO(2peak). A set of 211 transcripts was differentially expressed (ANOVA, P < 0.05 and fold change > 1.3). Functional enrichment analysis revealed that transcripts were mainly related to immune function, cell cycle processes, development, and growth. Baseline expression of 98 and 53 transcripts was associated with the absolute and relative (V) over dotO(2)peak response, respectively, with a strong correlation (r > 0.75, P < 0.01), and this panel was able to classify the 13 individuals according to their potential to improve oxygen uptake. A subset of 10 transcripts represented these signatures to a similar extent. PBMCs reveal a transcriptional signature responsive to endurance training. Additionally, a baseline transcriptional signature was associated with changes in (V) over dotO(2peak). Results might illustrate the possibility of obtaining molecular classifiers of endurance capacity changes through a minimally invasive blood sampling procedure. 47 2
13 23 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Zerbini Foundation Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FAPESP [2005/59740-7] CNPq [482863/2011-0, 483509/2012-4]