In this paper, we demonstrate for the first time the use of silver nanoparticles (AgNPs) for colorimetric ascorbic acid (AA) quantification in a paper-based sensor. This device is constituted by spot tests modified with AgNPs and silver ions bordered by a hydrophobic barrier which provides quantitative and fast analysis of AA. In addition, this device is employed as point-of-care monitoring using a unique drop of the sample. AgNPs paper-based sensor changed from light yellow to gray color after the addition of AA due to nanoparticle growth and clusters formation. The color intensities were altered as a function of AA concentration which were measured by either a scanner or a homemade portable transmittance colorimeter. Under the selected measurement conditions, results presented limit of detection which was comparable to analytical laboratory-based methodologies. In addition, the sensitivity of our sensor was comparable to the standard titration method when real samples were investigated. © 2015 Elsevier B.V.141 188194Devadasu, V.R., Bhardwaj, V., Kumar, M.N.V.R., (2013) Chem. Rev., 113, pp. 1686-1735Doria, G., Conde, J., Veigas, B., Giestas, L., Almeida, C., Assuncao, M., Rosa, J., Baptista, P.V., (2012) Sensors, 12, pp. 1657-1687Singh, A., Mody, H., Siwale, R., Mody, V., (2010) J. Pharm. Bioal. Sci., 2, pp. 282-289Gao, J., Gu, H., Xu, B., (2009) Acc. Chem. Res., 42, pp. 1097-1107Kalluri, J.R., Arbneshi, T., Khan, S.A., Neely, A., Candice, P., Varisli, B., Washington, M., Ray, P.C., (2009) Angew. Chem. Int. Ed., 48, pp. 9668-9671Jiang, X.C., Yu, A.B., (2008) Langmuir, 24, pp. 4300-4309Ko, S., Gunasekaran, S., Yu, J., (2010) Food Control, 21, pp. 155-161Ding, N., Cao, Q., Zhao, H., Yang, Y., Zeng, L., He, Y., Xiang, K., Wang, G., (2010) Sensors, 10, pp. 11144-11155Ornatska, M., Sharpe, E., Andreescu, D., Andreescu, S., (2011) Anal. Chem., 83, pp. 4273-4280Zhao, W., Ali, M.M., Aguirre, S.D., Brook, M.A., Li, Y., (2008) Anal. Chem., 80, pp. 8431-8437Li, L., Li, B., Cheng, D., Mao, L., (2010) Food Chem., 122, pp. 895-900Sherry, L.J., Jin, R., Mirkin, C.A., Schatz, G.C., Van Duyne, R.P., (2006) Nano Lett., 6, pp. 2060-2065Caro, C., Lopez-Cartes, C., Zaderenko, P., Mejias, J.A., (2008) J. Raman Spectrosc., 39, pp. 1162-1169Thompson, D.G., Enright, A., Faulds, K., Smith, W.E., Graham, D., (2008) Anal. Chem., 80, pp. 2805-2810Ling, J., Sang, Y., Huang, C.Z., (2008) J. Pharm. Biomed. Anal., 47, pp. 860-864Bosanac, L., Aabo, T., Bendix, P.M., Oddershede, L.B., (2008) Nano Lett., 8, pp. 1486-1491Kelly, K.L., Coronado, E., Zhao, L.L., Schatz, G.C., (2003) J. Phys. Chem. B, 107, pp. 668-677Chah, S., Hammond, M.R., Zare, R.N., (2005) Chem. Biol., 12, pp. 323-328Schofield, C.L., Haines, A.H., Field, R.A., Russell, D.A., (2006) Langmuir, 22, pp. 6707-6711Ratnarathorn, N., Chailapakul, O., Henry, C.S., Dungchai, W., (2012) Talanta, 99, pp. 552-557Elmore, A.R., (2005) Int. J.Toxicol., 24, pp. 103-117(2006) AOAC Official Methods of Analysis 967.21 Ascorbic Acid in Vitamin Preparations and Juices - 2,6-Dichloindophenol Titrimetric MethodGazdik, Z., Zitka, O., Petrlova, J., Adam, V., Zehnalek, J., Horna, A., Reznicek, V., Kizek, R., (2008) Sensors, 8, pp. 7097-7112Shaw, P.E., Wilson, C.W., (1982) J. Agric. Food Chem., 30, pp. 394-396Iwase, H., Ono, I., (1993) J. Chromatogr. A, 654, pp. 215-220Fenoll, J., Martinez, A., Hellin, P., Flores, P., (2011) Food Chem., 127, pp. 340-344Fung, Y.S., Mo, S.Y., (1992) Anal. Chim. Acta, 261, pp. 375-380O'Connell, P.J., Gormally, C., Pravda, M., Guilbault, G.G., (2001) Anal. Chim. Acta, 431, pp. 239-247Fornaro, A., Coichev, N., (1998) Quim. Nova, 21, pp. 642-650Paim, A.P.S., Kronka, E.A.M., Reis, B.F., Korn, M., (1998) Quim. Nova, 21, pp. 47-50Jungreis, E., (1997) Spot Test Analysis: Clinical, Environmental, Forensic, and Geochemical Applications, , 2nd ed. John Wiley & Sons, Inc. New YorkMartinez, A.W., Phillips, S.T., Carrilho, E., Thomas, S.W., III, Sindi, H., Whitesides, G.M., (2008) Anal. Chem., 80, pp. 3699-3707Jokerst, J.C., Adkins, J.A., Bisha, B., Mentele, M.M., Goodridge, L.D., Henry, C.S., (2012) Anal. Chem., 84, pp. 2900-2907Dal Lago, V., De Oliveira, L.F., Goncalves D. K, A., Kobarg, J., Cardoso, M.B., (2011) J. Mater. Chem., 21, pp. 12267-12273Carrilho, E., Martinez, A.W., Whitesides, G.M., (2009) Anal. Chem., 81, pp. 7091-7095Ellerbee, A.K., Phillips, S.T., Siegel, A.C., Mirica, K.A., Martinez, A.W., Striehl, P., Jain, N., Whitesides, G.M., (2009) Anal. Chem., 81, pp. 8447-8452Ilavsky, J., Jemian, P.R., (2009) J. Appl. Cryst., 42, pp. 347-353Beaucage, G., (1995) J. Appl. Cryst., 28, pp. 717-728Beaucage, G., (1996) J. Appl. Cryst., 29, pp. 134-146Ozyurek, M., Gungor, N., Baki, S., Guclu, K., Apak, R., (2012) Anal. Chem., 84, pp. 8052-8059De Oliveira, L.F., Goncalves D. J, O., Goncalves D. K, A., Kobarg, J., Cardoso, M.B., (2013) J. Biomed. Nanotechnol., 9, pp. 1817-1826Kapoor, S., Lawless, D., Kennepohl, P., Meisel, D., Serpone, N., (1994) Langmuir, 10, pp. 3018-3022Affonso De Oliveira, J.F., Cardoso, M.B., (2014) Langmuir, 30, pp. 4879-4886Jiang, Z.J., Liu, C.Y., (2003) J. Phys. Chem. B, 107, pp. 12411-12415Mi, C., Wang, T., Zeng, P., Zhao, S., Wang, N., Xu, S., (2013) Anal. Methods, 5, pp. 1463-1468Lin, K.-C., Yeh, P.-C., Chen, S.-M., (2012) Int. J. Electrochem. Sci., 7, pp. 12752-12763Han, D., Han, T., Shan, C., Ivaska, A., Niu, L., (2010) Electroanalysis, 22, pp. 2001-2008Keeley, G.P., O'Neill, A., McEvoy, N., Peltekis, N., Coleman, J.N., Duesberg, G.S., (2010) J. Mater. Chem., 20, pp. 7864-7869Moeslinger, T., Brunner, M., Volf, I., Spieckermann, P.G., (1995) Clin. Chem., 41, pp. 1177-1181