The presence of Enterococcus spp. in food poses a danger to public health due to its frequent association with various clinical infections. Pathogenicity in Enterococcus is multifactorial and complex, and stems from a sequence of virulence factors. The aim of this study was to evaluate the presence of phenotypic and genotypic determinants of virulence in Enterococcus spp. isolated from curd cheese. A total of 53 strains of Enterococcus spp. were analysed as to their susceptibility to antimicrobial agents, production of hemolysins, DNAse, thermonuclease, and gelatinase, and the profile of virulence-encoding genes. It was found that 75.5% of the strains were resistant to at least one of the nine antibiotics tested, 26.42% were resistant to two, and 3.77% to three antibiotics. The presence of vancomycin-resistance phenotypes was seen in 11.33% of the strains. Haemolytic activity was observed in 100% of strains and DNAse production in only 3.8%. There was no production of thermonuclease or gelatinase. Strains resistant to vancomycin and teicoplanin were identified as E. faecium and Enterococcus spp. The profile of the genetic determinants of virulence was highly variable, and 90% of the strains harboured at least one of the nine genes being studied. The efaA gene showed the highest prevalence (70%), followed by the ace gene (50%), the esp gene and gelE gene (40%).4716976Ahmadova, A., Evaluation of antimicrobial activity, probiotic properties and safety of wild strain Enterococcus faecium AQ71 isolated from Azerbaijani Motal cheese (2013) Food Control, 30 (2), pp. 631-641(2005) Normas de desempenho para testes de sensibilidade antimicrobiana: 150 suplemento informativo, , http://www.anvisa.gov.br/servicosaude/manuais/clsi/clsi_OPASM100S15.pdf, Pennsylvania. (Document, M100-S15). Acesso em: 03 jul. 2014(2003) Padronização dos testes de sensibilidade a antimicrobianos por disco-difusão: norma aprovada-oitava edição, , http://www.anvisa.gov.br/servicosaude/manuais/clsi/clsi_OPASM2-A8.pdf, Pennsylvania. (CLSI Document, M2-A8). Acesso em: 19 ago. 2014Courvalin, P., Vancomycin resistance in Gram-positive cocci (2006) Clinical Infectious Diseases, 42 (1), pp. 25-34Cruz, T.E.E., Torres, J.M.O., (2012) Gelatin hydrolysis test protocol, , http://www.microbelibrary.org/library/laboratory-test/3776-gelatinhydrolysis-test-protocol, Washington: Microbial Library American Society for Microbiology. Acesso em: 19 ago. 2014Dogan, B., Distribution of serotypes and antimicrobial resistance genes among Streptococcus agalactiae isolates from bovine and human hosts (2005) Journal of Clinical Microbiology, 43 (12), pp. 5899-5906Dolci, P., Microbiological characterization of artisanal Raschera PDO cheese: analysis of its indigenous lactic acid bacteria (2008) Food Microbiology, 25 (2), pp. 392-399Dutka-Malen, S., Evers, S., Courvalin, P., Detection of glycopeptide resistance genotypes and identifications the species level of clinically relevant enterococci by PCR (1995) Journal of Clinical Microbiology, 33 (1), pp. 24-27Eaton, T.J., Gasson, M.J., Molecular screening of Enterococcus virulence determinants and potential genetic exchange between food and medical isolates (2001) Applied and Environmental Microbiology, 67 (4), pp. 1628-1635Foulquié-Moreno, M.R., The role and application of enterococci in food and health (2006) International Journal of Food Microbiology, 106 (1), pp. 1-24Franz, C.M.A.P., Holzapfel, W.H., Enterococci (2006) Emerging foodborne pathogens, pp. 557-613. , MOTARJEMI, Y.ADAMS, M. New York: CRC Press. cap. 20Gomes, B.C., Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods (2008) Food Microbiology, 25 (5), pp. 668-675Hasan, A.A., Molecular and biochemical identification of coagulase positive Staphylococcus species isolated from human and animal sources in Jordan (2014) International Journal of Medicine and Medical Sciences, 47 (1), pp. 1491-1507Jamet, E., Prevalence and characterization of antibiotic resistant Enterococcus faecalis in French cheeses (2012) Food Microbiology, 31 (2), pp. 191-198Johansson, D., Rasmussen, M., Virulence factors in isolates of Enterococcus faecalis from infective endocarditis and from the normal flora (2013) Microbial Pathogenesis, 55, pp. 28-31(1998), http://www.bacterio.net/-allnames.html, Acesso em: 20 jan. 2015Lopes, M.F.S., Activity and expression of a virulence factor, gelatinase, in dairy enterococci (2006) International Journal of Food Microbiology, 112 (3), pp. 208-214Mac, K., Species identification and detection of vancomycin resistance genes in enterococci of animal origin by multiplex PCR (2003) International Journal of Food Microbiology, 88 (2-3), pp. 305-309Malek, R., Technological and safety properties display biodiversity among enterococci isolated from two Egyptian cheeses, "Ras" and "Domiati" (2012) International Journal of Food Microbiology, 153 (3), pp. 314-322Mannu, L., Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin (2003) International Journal of Food Microbiology, 88 (2-3), pp. 291-304Marguet, E.R., Vallejo, M., Olivera, N.L., Factores de virulencia de cepas de Enterococcus aisladas de quesos ovinos (2008) Acta Bioquímica Clínica Latinoamericana, 42 (4), pp. 543-548Marques, E.B., Suzart, S., Occurrence of virulenceassociated genes in clinical Enterococcus faecalis strains isolated in Londrina, Brazil (2004) Journal of Medical Microbiology, 53 (11), pp. 1069-1073Martín, B., Garriga, M., Aymerich, M., Identification of Enterococcus species by melting curve analysis of restriction fragments (2008) Journal of Microbiological Methods, 75 (1), pp. 145-147Moraes, P.M., Bacteriocinogenic and virulence potential of Enterococcus isolates obtained from raw milk and cheese (2012) Journal of Applied Microbiology, 113 (2), pp. 318-328Morandi, S., Silvetti, T., Brasca, M., Biotechnological and safety characterization of Enterococcus lactis, a recently described species of dairy origin (2013) Antonie Van Leeuwenhoek, 103 (1), pp. 239-249Moura, R., A consolidação do queijo de coalho (2012) Agroindústria Tropical, (140), pp. 5-12Riboldi, G.P., Antimicrobial resistance profile of Enterococcus spp. isolated from food in southern Brazil (2009) Brazilian Journal of Microbiology, 40 (1), pp. 125-128Sánchez-Porro, C., Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes (2003) Journal Applied Microbiology, 94 (2), pp. 295-300Santos, K.M.O., Brazilian artisanal cheeses as a source of beneficial Enterococcus faecium strains: characterization of the bacteriocinogenic potential (2014) Annals of Microbiology, 64 (4), pp. 1463-1471Yogurtcu, N.N., Tuncer, Y., Antibiotic susceptibility patterns of Enterococcus strains isolated from Turkish Tulum cheese (2013) International Journal of Dairy Technology, 66 (2), pp. 236-242