Low-level Laser Therapy (lllt) In Dystrophin-deficient Muscle Cells: Effects On Regeneration Capacity, Inflammation Response And Oxidative Stress

dc.date2015
dc.date2016-06-03T20:14:39Z
dc.date2016-06-03T20:14:39Z
dc.date.accessioned2018-03-29T01:33:21Z
dc.date.available2018-03-29T01:33:21Z
dc.identifier
dc.identifierPlos One. Public Library Of Science, v. 10, n. 6, p. , 2015.
dc.identifier19326203
dc.identifier10.1371/journal.pone.0128567
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84939132902&partnerID=40&md5=1c5f0bc22f5abd6c55fa954cc8b480d1
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/238246
dc.identifier2-s2.0-84939132902
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1304907
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe present study evaluated low-level laser therapy (LLLT) effects on some physiological pathways that may lead to muscle damage or regeneration capacity in dystrophin-deficient muscle cells of mdx mice, the experimental model of Duchenne muscular dystrophy (DMD). Primary cultures of mdx skeletal muscle cells were irradiated only one time with laser and analyzed after 24 and 48 hours. The LLLT parameter used was 830 nm wavelengths at 5 J/cm2fluence. The following groups were set up: Ctrl (untreated C57BL/10 primary muscle cells), mdx (untreated mdx primary muscle cells), mdx LA 24 (mdx primary muscle cells - LLLT irradiated and analyzed after 24 h), and mdx LA 48 (mdx primary muscle cells - LLLT irradiated and analyzed after 48 h). The mdx LA 24 and mdx LA 48 groups showed significant increase in cell proliferation, higher diameter in muscle cells and decreased MyoD levels compared to the mdx group. The mdx LA 48 group showed significant increase in Myosin Heavy Chain levels compared to the untreated mdx and mdx LA 24 groups. The mdx LA 24 and mdx LA 48 groups showed significant increase in [Ca2+]i. The mdx group showed significant increase in H<inf>2</inf>O<inf>2</inf> production and 4-HNE levels compared to the Ctrl group and LLLT treatment reduced this increase. GSH levels and GPx, GR and SOD activities increased in the mdx group. Laser treatment reduced the GSH levels and GR and SOD activities in dystrophic muscle cells. The mdx group showed significant increase in the TNF-α and NF-κB levels, which in turn was reduced by the LLLT treatment. Together, these results suggest that the laser treatment improved regenerative capacity and decreased inflammatory response and oxidative stress in dystrophic muscle cells, indicating that LLLT could be a helpful alternative therapy to be associated with other treatment for dystrophinopathies. © 2015 Macedo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description10
dc.description6
dc.description
dc.description
dc.descriptionCAPES, Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.descriptionCNPq, Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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dc.description
dc.description
dc.languageen
dc.publisherPublic Library of Science
dc.relationPLoS ONE
dc.rightsaberto
dc.sourceScopus
dc.titleLow-level Laser Therapy (lllt) In Dystrophin-deficient Muscle Cells: Effects On Regeneration Capacity, Inflammation Response And Oxidative Stress
dc.typeArtículos de revistas


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