A positive outlook regarding future events, in which individuals find themselves less likely than others to experience negative events, is called optimistic bias (OB). The aims of this study were: 1. To ascertain the existence and measure the OB phenomenon in food handlers in relation to foodborne disease and 2. To examine the association of OB with food handlers' risk perceptions, knowledge, attitudes, self-reported practices, training participation, food safety performance of the establishments and microbiological analysis. This study examined different food businesses in Santos city, in Brazil, as follows: street food kiosks, beach kiosks, restaurants, hospitals, and school meal services. The food handlers indicated their own risk and their peers' risk of causing a foodborne disease. Responses were given on a 10. cm linear scale anchored with descriptors of intensity ranging from "none" to "very high". The difference between these risk perceptions characterized a score of tendency of an OB. A structured questionnaire was administered to evaluate knowledge, attitudes, self-reported practices and food safety performance. A total of 183 food handlers participated in the study. Microbiological analyses were conducted with ready-to-eat foods/preparations from selected sites. The food handlers perceived themselves as less likely than their peers to cause a foodborne disease (p < 0.001), demonstrating the tendency of an OB. Food handlers who had undergone some training presented lower scores for the perceived risk of themselves being responsible for a foodborne disease and higher knowledge than did untrained individuals. Hospitals, schools and restaurants performed better than street food group considering food safety performance. However, microorganisms were found in food samples from hospitals. This result may be motivated by OB and other subjective factors. Apparently, this positive outlook of food handlers is associated with training participation, lethality perception and correlating positively with age. No association was found between OB scores and knowledge, attitudes and self-reported practices. Since OB may lead individuals to adopt inappropriate or dangerous behavior, strategies for debiasing food handlers should be designed. © 2015 Elsevier Ltd.75 2733Bennett, R.W., Belay, N., Bacillus cereus (1981) Compendium of Methods for the Microbiological Examination of Foods, pp. 311-316. , American Public Health Association, Washington, DC, F.P. Downes, K. Ito (Eds.)(2001) Resolution RDC n° 12, of 02 January 2001 - Approves the Technical Regulation on microbiological standards for foods, , (Brasília - Brazil)Resolution no. 216 of the 15th of September 2004 (2004) Establishes the technical regulation of good manufacturing practices to foodservicesChock, T.M., The influence of body mass index, sex, and race on college students' optimistic bias for lifestyle healthfulness (2011) Journal of Nutrition Education and Behavior, 43 (5), pp. 331-338Choudhury, M., Mahanta, L.B., Goswami, J.S., Mazumder, M.D., Will capacity building training interventions given to street food vendors give us safer food?: A cross-sectional study from India (2011) Food Control, 22 (8), pp. 1233-1239Claro, R.M., Baraldi, L.G., Martins, A.P.B., Bandoni, D.H., Levy, R.B., Evolução das despesas com alimentação fora do domicílio e influência da renda no Brasil, 2002/2003 a 2008/2009 (2014) Cadernos de Saúde Pública, 30 (7), pp. 1418-1426Recommended international code of practice general principles of food hygiene (2003) CAC/RCP 1-1969Costalunga, S., Tondo, E.C., Salmonellosis in Rio Grande do Sul, Brazil, 1997 to 1999 (2002) Brazilian Journal of Microbiology, 33, p. 5Da Cunha, D.T., Stedefeldt, E., De Rosso, V.V., He is worse than I am: The positive outlook of food handlers about foodborne disease (2014) Food Quality and Preference, 35 (7), pp. 95-97Da Cunha, D.T., Stedefeldt, E., De Rosso, V.V., The role of theoretical food safety training on Brazilian food handlers' knowledge, attitude and practice (2014) Food Control, 43 (9), pp. 167-174De Oliveira, A.B.A., Da Cunha, D.T., Stedefeldt, E., Capalonga, R., Tondo, E.C., Cardoso, M.R.I., Hygiene and good practices in school meal services: Organic matter on surfaces, microorganisms and health risks (2014) Food Control, 40, pp. 120-126Downes, F.P., Ito, K., (2001) Compendium of methods for the microbiological examination of foods, p. 676. , American, Public Health Association (APHA), WashingtonEgan, M.B., Raats, M.M., Grubb, S.M., Eves, A., Lumbers, M.L., Dean, M.S., A review of food safety and food hygiene training studies in the commercial sector (2007) Food Control, 18 (10), pp. 1180-1190(2008) Annual summary of outbreaks in New Zealand 2007, p. 28. , Population and Environmental Health Group, New Zealand(2009) FDA report on the occurrence of foodborne illness risk factors in selected institutional foodservice, restaurant, and retail food store facility types, , http://www.fda.gov/downloads/Food/FoodSafety/RetailFoodProtection/FoodborneIllnessandRiskFactorReduction/RetailFoodRiskFactorStudies/ucm123546.pdf, (United States of America, Available at:, FDA Retail Food Program Steering Committee (Ed.)Gouveia, S.O., Clarke, V., Optimistic bias for negative and positive events (2001) Health Education, 101 (5), pp. 228-234Green, L., Selman, C., Banerjee, A., Marcus, R., Medus, C., Angulo, F.J., Food service workers' self-reported food preparation practices: an EHS-Net study (2005) International Journal of Hygiene and Environmental Health, 208 (1-2), pp. 27-35Guthrie, J.F., Lin, B.H., Frazao, E., Role of food prepared away from home in the American diet, 1977-78 versus 1994-96: Changes and consequences (2002) Journal of Nutrition Education and Behavior, 34 (3), pp. 140-150Helweg-Larsen, M., Shepperd, J.A., Do moderators of the optimistic bias affect personal or target risk estimates? A review of the literature (2001) Personality and Social Psychology Review, 5 (1), pp. 74-95Horswill, M.S., McKenna, F.P., The effect of perceived control on risk taking (1999) Journal of Applied Social Psychology, 29 (2), pp. 377-391Howes, M., McEwan, S., Griffiths, M., Harris, L., Food handler certification by home study: Measuring changes in knowledge and behaviour (1996) Dairy, Food and Environmental Sanitation, 16 (11), pp. 737-744Janz, N.K., Becker, M.H., The health belief model - A decade later (1984) Health Education Quarterly, 11 (1), pp. 1-47Kjeldgaard, K.J., Stormly, M.L., Leisner, J.J., Relation between microbial levels of ready-to-eat foods and the monitoring of compliance with HACCP-based own control programs in small Danish food outlets (2010) Food Control, 21 (11), pp. 1453-1457Ko, W.H., The relationship among food safety knowledge, attitudes and self-reported HACCP practices in restaurant employees (2013) Food Control, 29 (1), pp. 192-197Kornacki, J.L., Johnson, J.L., Enterobateriaceae, coliforms and Escherichia coli as quality and safety indicators (2001) Compendium of methods for the microbiological examination of foods, pp. 69-82. , APHA - American Public Health Association, Washington, C. Vanderzant, D.F. Splittstoesser (Eds.)Lima, S.C., Loiko, M., Casarin, L.S., Tondo, E.C., Assessing the epidemiological data of Staphylococcus aureus food poisoning occurred in Rio Grande do Sul (2013) Southern Brazil, Brazilian Journal of Microbiology, 44 (3), pp. 759-763McIntyre, L., Vallaster, L., Wilcott, L., Henderson, S.B., Kosatsky, T., Evaluation of food safety knowledge, attitudes and self-reported hand washing practices in FOODSAFE trained and untrained food handlers in British Columbia, Canada (2013) Food Control, 30 (1), pp. 150-156Medeiros, C.O., Cavalli, S.B., Salay, E., Proença, R.P.C., Assessment of the methodological strategies adopted by food safety training programmes for food service workers: A systematic review (2011) Food Control, 22, pp. 1136-1144Miles, S., Braxton, D.S., Frewer, L.J., Public perceptions about microbiological hazards in food (1999) British Food Journal, 101 (10), pp. 744-762Miles, S., Frewer, L.J., Investigating specific concerns about different food hazards (2001) Food Quality and Preference, 12 (1), pp. 47-61Miles, S., Scaife, V., Optimistic bias and food (2003) Nutrition Research Reviews, 16 (1), pp. 3-19Park, S.H., Kwak, T.K., Chang, H.J., Evaluation of the food safety training for food handlers in restaurants operations (2012) Nutrition Practice and Research, 4 (1), pp. 58-68(2011) R: A language and environment for statistical computing, , http://www.R-project.org/, R Foundation for Statistical Computing, Vienna - Austria, (Available at:Rane, S., Street vended food in developing world: Hazard analyses (2011) Indian Journal of Microbiology, 51 (1), pp. 100-106Rennie, D.M., Evaluation of food hygiene education (1994) British Food Journal, 96 (11), pp. 20-25Seaman, P., Eves, A., Food hygiene training in small to medium-sized care settings (2008) International Journal of Environmental Health Research, 18 (5), pp. 365-374Shepperd, J.A., McNulty, J.K., The affective consequences of expected and unexpected outcomes (2002) Psychological Science, 13 (1), pp. 85-88Sjoberg, L., Factors in risk perception (2000) Risk Analysis, 20 (1), pp. 1-11Taylor, K.M., Shepperd, J.A., Bracing for the worst: Severity, testing, and feedback timing as moderators of the optimistic bias (1998) Personality and Social Psychology Bulletin, 24 (9), pp. 915-926Tebbutt, G.M., Southwell, M., Compliance with recent food hygiene legislation and microbiological monitoring in cooked meat product plants (1997) International Journal of Environmental Health Research, 7 (4), pp. 335-344Todd, E.C.D., Greig, J.D., Bartleson, C.A., Michaels, B.S., Outbreaks where food workers have been implicated in the spread of foodborne disease. Part 3. Factors contributing to outbreaks and description of outbreak categories (2007) Journal of Food Protection, 70 (9), pp. 2199-2217Weinstein, N.D., Unrealistic optimism about future life events (1980) Journal of Personality and Social Psychology, 39 (5), pp. 806-820Weinstein, N.D., Unrealistic optimism about susceptibility to health-problems (1982) Journal of Behavioral Medicine, 5 (4), pp. 441-460Weinstein, N.D., Why it wont happen to me - perceptions of risk-factors and susceptibility (1984) Health Psychology, 3 (5), pp. 431-457Weinstein, N.D., Unrealistic optimism about susceptibility to health-problems - Conclusions from a community-wide sample (1987) Journal of Behavioral Medicine, 10 (5), pp. 481-500Weinstein, N.D., Optimistic biases about personal risks (1989) Science, 246 (4935), pp. 1232-1233Weinstein, N.D., Klein, W.M., Resistance of personal risk perceptions to debiasing interventions (1995) Health Psychology, 14 (2), pp. 132-140Xue, J.H., Zhang, W.J., Understanding China's food safety problem: An analysis of 2387 incidents of acute foodborne illness (2013) Food Control, 30 (1), pp. 311-317