Tissue Microarray Use For Immunohistochemical Study Of Ameloblastoma.

dc.creatorNeves-Silva, Rodrigo
dc.creatorFonseca, Felipe Paiva
dc.creatorde Jesus, Adriana Souza
dc.creatorPontes, Hélder Antônio Rebelo
dc.creatorRocha, André Caroli
dc.creatorBrandão, Thais Bianca
dc.creatorVargas, Pablo Agustin
dc.creatorLopes, Márcio Ajudarte
dc.creatorde Almeida, Oslei Paes
dc.creatorSantos-Silva, Alan Roger
dc.date2016-Feb
dc.date2016-05-23T19:43:45Z
dc.date2016-05-23T19:43:45Z
dc.date.accessioned2018-03-29T01:30:52Z
dc.date.available2018-03-29T01:30:52Z
dc.identifierJournal Of Oral Pathology & Medicine : Official Publication Of The International Association Of Oral Pathologists And The American Academy Of Oral Pathology. , 2016-Feb.
dc.identifier1600-0714
dc.identifier10.1111/jop.12428
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/26841348
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/236047
dc.identifier26841348
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1304290
dc.descriptionAmeloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different human neoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. Forty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0- and 2.0-mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl-2 and Ki-67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole-section slides (CWSS). Kappa statistical test demonstrated that both 1.0- and 2.0-mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0- and 2.0-mm cores, and between duplicate and triplicate values. 1.0-mm TMA showed a higher index of core loss (33.74% vs. 4.99%). Using a manual arrayer, it was demonstrated that 1.0-mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS.
dc.description
dc.description
dc.languageeng
dc.relationJournal Of Oral Pathology & Medicine : Official Publication Of The International Association Of Oral Pathologists And The American Academy Of Oral Pathology
dc.relationJ. Oral Pathol. Med.
dc.rightsembargo
dc.sourcePubMed
dc.subjectBcl-2
dc.subjectKi67
dc.subjectAmeloblastoma
dc.subjectCytokeratin
dc.subjectTissue Microarray
dc.titleTissue Microarray Use For Immunohistochemical Study Of Ameloblastoma.
dc.typeArtículos de revistas


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