Improving The Differential Diagnosis Between Myelodysplastic Syndromes And Reactive Peripheral Cytopenias By Multiparametric Flow Cytometry: The Role Of B-cell Precursors.

dc.creatorReis-Alves, Suiellen C
dc.creatorTraina, Fabiola
dc.creatorMetze, Konradin
dc.creatorLorand-Metze, Irene
dc.date2015
dc.date2016-05-23T19:43:03Z
dc.date2016-05-23T19:43:03Z
dc.date.accessioned2018-03-29T01:30:14Z
dc.date.available2018-03-29T01:30:14Z
dc.identifierDiagnostic Pathology. v. 10, p. 44, 2015.
dc.identifier1746-1596
dc.identifier10.1186/s13000-015-0259-3
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25924846
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/235878
dc.identifier25924846
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1304121
dc.descriptionImmunophenotyping is a valuable ancillary technique for the differential diagnosis between myelodysplastic syndromes (MDS) with low bone marrow (BM) blast counts and a normal karyotype, and reactive peripheral (PB) cytopenias. Our aim was to search for the most important variables for this purpose. We also analyzed the age variation of BM B-cell precursors (BCP) and its differences in reactive and clonal cytopenias. Immunophenotypic analyzes were performed in BM of 54 patients with MDS (76% with BM blasts <5%) and 35 cases of reactive cytopenias. Healthy allogeneic BM transplantation donors (n = 41) were used as controls. We used a four-color panel of antibodies analyzing 9 granulocytic, 8 monocytic and 6 CD34(+) cell features. Asynchronous shift to the left in maturing granulocytes and increase in CD16(+) monocytes were also found in reactive PB cytopenias, but the most important aberrancies in MDS were seen in myeloid CD34(+) cells. Decrease in BCP, that is a hallmark of MDS, could also be found in reactive cytopenias, especially in patients >55 years. % BM BCP could be calculated by the formula: (-7.97 × log age) + (4.24 × log % CD34 (+) cells) - (0.22 x nr. alterations CD34 (+) cells) + 0.577. Corrected R(2) = 0.467. Analysis of myelomonocytic precursors and CD34(+) cells was satisfactory for the differential diagnosis between reactive PB cytopenias and MDS. The most specific alterations were found in CD34(+) cells. Comparison of the values obtained with those of normal age-matched controls is recommended. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1975931809154663.
dc.description10
dc.description44
dc.languageeng
dc.relationDiagnostic Pathology
dc.relationDiagn Pathol
dc.rightsaberto
dc.sourcePubMed
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAge Factors
dc.subjectAged
dc.subjectAged, 80 And Over
dc.subjectAntigens, Cd34
dc.subjectBiomarkers
dc.subjectCase-control Studies
dc.subjectDiagnosis, Differential
dc.subjectFemale
dc.subjectFlow Cytometry
dc.subjectHumans
dc.subjectImmunophenotyping
dc.subjectKaryotyping
dc.subjectLeukopenia
dc.subjectLymphocyte Count
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMyelodysplastic Syndromes
dc.subjectPrecursor Cells, B-lymphoid
dc.subjectPredictive Value Of Tests
dc.subjectYoung Adult
dc.titleImproving The Differential Diagnosis Between Myelodysplastic Syndromes And Reactive Peripheral Cytopenias By Multiparametric Flow Cytometry: The Role Of B-cell Precursors.
dc.typeArtículos de revistas


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