Changes In Calsequestrin, Tnf-α, Tgf-β And Myod Levels During The Progression Of Skeletal Muscle Dystrophy In Mdx Mice: A Comparative Analysis Of The Quadriceps, Diaphragm And Intrinsic Laryngeal Muscles.

dc.creatorBarros Maranhão, Juliana
dc.creatorde Oliveira Moreira, Drielen
dc.creatorMaurício, Adriana Fogagnolo
dc.creatorde Carvalho, Samara Camaçari
dc.creatorFerretti, Renato
dc.creatorPereira, Juliano Alves
dc.creatorSanto Neto, Humberto
dc.creatorMarques, Maria Julia
dc.date2015-Oct
dc.date2016-05-23T19:40:30Z
dc.date2016-05-23T19:40:30Z
dc.date.accessioned2018-03-29T01:28:03Z
dc.date.available2018-03-29T01:28:03Z
dc.identifierInternational Journal Of Experimental Pathology. v. 96, n. 5, p. 285-293, 2015-Oct.
dc.identifier1365-2613
dc.identifier10.1111/iep.12142
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/26515458
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/235305
dc.identifier26515458
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1303548
dc.descriptionIn Duchenne muscular dystrophy (DMD), the search for new biomarkers to follow the evolution of the disease is of fundamental importance in the light of the evolving gene and pharmacological therapies. In addition to the lack of dystrophin, secondary events including changes in calcium levels, inflammation and fibrosis greatly contribute to DMD progression and the molecules involved in these events may represent potential biomarkers. In this study, we performed a comparative evaluation of the progression of dystrophy within muscles that are differently affected by dystrophy (diaphragm; DIA and quadriceps; QDR) or spared (intrinsic laryngeal muscles) using the mdx mice model of DMD. We assessed muscle levels of calsequestrin (calcium-related protein), tumour necrosis factor (TNF-α; pro-inflammatory cytokine), tumour growth factor (TGF-β; pro-fibrotic factor) and MyoD (muscle proliferation) vs. histopathology at early (1 and 4 months of age) and late (9 months of age) stages of dystrophy. Fibrosis was the primary feature in the DIA of mdx mice (9 months: 32% fibrosis), which was greater than in the QDR (9 months: 0.6% fibrosis). Muscle regeneration was the primary feature in the QDR (9 months: 90% of centrally nucleated fibres areas vs. 33% in the DIA). The QDR expressed higher levels of calsequestrin than the DIA. Laryngeal muscles showed normal levels of TNF-α, TGF-β and MyoD. A positive correlation between histopathology and cytokine levels was observed only in the diaphragm, suggesting that TNF-α and TGF-β serve as markers of dystrophy primarily for the diaphragm.
dc.description96
dc.description285-293
dc.languageeng
dc.relationInternational Journal Of Experimental Pathology
dc.relationInt J Exp Pathol
dc.rightsembargo
dc.sourcePubMed
dc.subjectCalsequestrin
dc.subjectDystrophy
dc.subjectMdx
dc.subjectTumour Growth Factor-β
dc.subjectTumour Necrosis Factor-α
dc.titleChanges In Calsequestrin, Tnf-α, Tgf-β And Myod Levels During The Progression Of Skeletal Muscle Dystrophy In Mdx Mice: A Comparative Analysis Of The Quadriceps, Diaphragm And Intrinsic Laryngeal Muscles.
dc.typeArtículos de revistas


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