Cromatografia de afinidade por íons metálicos imobilizados (IMAC) de biomoléculas: aspectos fundamentais e aplicações tecnológicas
Immobilized metal-ion affinity chromatography (IMAC) of biomolecules: fundamental aspects and technological applications
| dc.creator | Bresolin, Igor Tadeu Lazzarotto | |
| dc.creator | Miranda, Everson Alves | |
| dc.creator | Bueno, Sônia Maria Alves | |
| dc.date | 2009-01-01 | |
| dc.date | 2015-12-03T13:30:52Z | |
| dc.date | 2015-12-03T13:30:52Z | |
| dc.date.accessioned | 2018-03-29T01:24:53Z | |
| dc.date.available | 2018-03-29T01:24:53Z | |
| dc.identifier | Química Nova. Sociedade Brasileira de Química, v. 32, n. 5, p. 1288-1296, 2009. | |
| dc.identifier | 0100-4042 | |
| dc.identifier | S0100-40422009000500035 | |
| dc.identifier | 10.1590/S0100-40422009000500035 | |
| dc.identifier | http://dx.doi.org/10.1590/S0100-40422009000500035 | |
| dc.identifier | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000500035 | |
| dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/23853 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/202475 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1302708 | |
| dc.description | Immobilized Metal Ion Affinity Cromatography - IMAC - is a group-specific based adsorption applied to the purification and structure-function studies of proteins and nucleic acids. The adsorption is based on coordination between a metal ion chelated on the surface of a solid matrix and electron donor groups at the surface of the biomolecule. IMAC is a highly selective, low cost, and easily scaled-up technique being used in research and commercial operations. A separation process can be designed for a specific molecule by just selecting an appropriate metal ion, chelating agent, and operational conditions such as pH, ionic strength, and buffer type. | |
| dc.description | 1288 | |
| dc.description | 1296 | |
| dc.language | pt | |
| dc.publisher | Sociedade Brasileira de Química | |
| dc.relation | Química Nova | |
| dc.rights | aberto | |
| dc.source | SciELO | |
| dc.subject | immobilized metal-ion affinity chromatography | |
| dc.subject | purification | |
| dc.subject | biomolecules | |
| dc.title | Cromatografia de afinidade por íons metálicos imobilizados (IMAC) de biomoléculas: aspectos fundamentais e aplicações tecnológicas | |
| dc.title | Immobilized metal-ion affinity chromatography (IMAC) of biomolecules: fundamental aspects and technological applications | |
| dc.type | Artículos de revistas |