Application Of Laser Microdissection Icp-ms For High Resolution Elemental Mapping In Mouse Brain Tissue: A Comparative Study With Laser Ablation Icp-ms.

dc.creatorSussulini, Alessandra
dc.creatorBecker, J Sabine
dc.date2015-Jan
dc.date2015-11-27T13:46:45Z
dc.date2015-11-27T13:46:45Z
dc.date.accessioned2018-03-29T01:24:16Z
dc.date.available2018-03-29T01:24:16Z
dc.identifierTalanta. v. 132, p. 579-82, 2015-Jan.
dc.identifier1873-3573
dc.identifier10.1016/j.talanta.2014.10.001
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25476347
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/202319
dc.identifier25476347
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1302552
dc.descriptionMapping of elements in biological tissue by laser induced mass spectrometry is a fast growing analytical methodology in life sciences. This method provides a multitude of useful information of metal, nonmetal, metalloid and isotopic distribution at major, minor and trace concentration ranges, usually with a lateral resolution of 12-160 µm. Selected applications in medical research require an improved lateral resolution of laser induced mass spectrometric technique at the low micrometre scale and below. The present work demonstrates the applicability of a recently developed analytical methodology - laser microdissection associated to inductively coupled plasma mass spectrometry (LMD ICP-MS) - to obtain elemental images of different solid biological samples at high lateral resolution. LMD ICP-MS images of mouse brain tissue samples stained with uranium and native are shown, and a direct comparison of LMD and laser ablation (LA) ICP-MS imaging methodologies, in terms of elemental quantification, is performed.
dc.description132
dc.description579-82
dc.languageeng
dc.relationTalanta
dc.relationTalanta
dc.rightsfechado
dc.rightsCopyright © 2014 Elsevier B.V. All rights reserved.
dc.sourcePubMed
dc.subjectInductively Coupled Plasma Mass Spectrometry
dc.subjectLaser Ablation
dc.subjectLaser Microdissection
dc.subjectMass Spectrometry Imaging
dc.subjectMouse Brain
dc.subjectQuantification
dc.titleApplication Of Laser Microdissection Icp-ms For High Resolution Elemental Mapping In Mouse Brain Tissue: A Comparative Study With Laser Ablation Icp-ms.
dc.typeArtículos de revistas


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