Increased Expression Of Hes5 Protein In Notch Signaling Pathway In The Hippocampus Of Mice Offspring Of Dams Fed A High-fat Diet During Pregnancy And Suckling.

dc.creatorMendes-da-Silva, Cristiano
dc.creatorLemes, Simone Ferreira
dc.creatorBaliani, Tanyara da Silva
dc.creatorVersutti, Milena Diorio
dc.creatorTorsoni, Marcio Alberto
dc.date2015-Feb
dc.date2015-11-27T13:46:42Z
dc.date2015-11-27T13:46:42Z
dc.date.accessioned2018-03-29T01:24:12Z
dc.date.available2018-03-29T01:24:12Z
dc.identifierInternational Journal Of Developmental Neuroscience : The Official Journal Of The International Society For Developmental Neuroscience. v. 40, p. 35-42, 2015-Feb.
dc.identifier1873-474X
dc.identifier10.1016/j.ijdevneu.2014.11.005
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25450527
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/202302
dc.identifier25450527
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1302535
dc.descriptionMaternal high-fat diet (HFD) impairs hippocampal development of offspring promoting decreased proliferation of neural progenitors, in neuronal differentiation, in dendritic spine density and synaptic plasticity reducing neurogenic capacity. Notch signaling pathway participates in molecular mechanisms of the neurogenesis. The activation of Notch signaling leads to the upregulation of Hes5, which inhibits the proliferation and differentiation of neural progenitors. This study aimed to investigate the Notch/Hes pathway activation in the hippocampus of the offspring of dams fed an HFD. Female Swiss mice were fed a control diet (CD) and an HFD from pre-mating until suckling. The bodyweight and mass of adipose tissue in the mothers and pups were also measured. The mRNA and protein expression of Notch1, Hes5, Mash1, and Delta1 in the hippocampus was assessed by RT-PCR and western blotting, respectively. Dams fed the HFD and their pups had an increased bodyweight and amount of adipose tissue. Furthermore, the offspring of mothers fed the HFD exhibited an increased Hes5 expression in the hippocampus compared with CD offspring. In addition, HFD offspring also expressed increased amounts of Notch1 and Hes5 mRNA, whereas Mash1 expression was decreased. However, the expression of Delta1 did not change significantly. We propose that the overexpression of Hes5, a Notch effector, downregulates the expression of the proneural gene Mash1 in the offspring of obese mothers, delaying cellular differentiation. These results provide further evidence that an offspring's hippocampus is molecularly susceptible to maternal HFD and suggest that Notch1 signaling in this brain region is important for neuronal differentiation.
dc.description40
dc.description35-42
dc.languageeng
dc.relationInternational Journal Of Developmental Neuroscience : The Official Journal Of The International Society For Developmental Neuroscience
dc.relationInt. J. Dev. Neurosci.
dc.rightsfechado
dc.rightsCopyright © 2014 ISDN. Published by Elsevier Ltd. All rights reserved.
dc.sourcePubMed
dc.subjectHigh-fat Diet
dc.subjectHippocampus
dc.subjectLactation
dc.subjectMaternal Obesity
dc.subjectNotch Signaling
dc.subjectPregnancy
dc.titleIncreased Expression Of Hes5 Protein In Notch Signaling Pathway In The Hippocampus Of Mice Offspring Of Dams Fed A High-fat Diet During Pregnancy And Suckling.
dc.typeArtículos de revistas


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