Towards A Deeper Understanding Of Structural Biomass Recalcitrance Using Phase-contrast Tomography.

dc.creatorIsaac, Augusta
dc.creatorBarboza, Vinicius
dc.creatorSket, Federico Ivan
dc.creatorD'Almeida, José Roberto M
dc.creatorMontoro, Luciano Andrey
dc.creatorHilger, André
dc.creatorManke, Ingo
dc.date2015
dc.date2015-11-27T13:46:29Z
dc.date2015-11-27T13:46:29Z
dc.date.accessioned2018-03-29T01:23:57Z
dc.date.available2018-03-29T01:23:57Z
dc.identifierBiotechnology For Biofuels. v. 8, p. 40, 2015.
dc.identifier1754-6834
dc.identifier10.1186/s13068-015-0229-8
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25788981
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/202234
dc.identifier25788981
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1302467
dc.descriptionThe development of technological routes to convert lignocellulosic biomass to liquid fuels requires an in-depth understanding of the cell wall architecture of substrates. Essential pretreatment processes are conducted to reduce biomass recalcitrance and usually increase the reactive surface area. Quantitative three-dimensional information about both bulk and surface structural features of substrates needs to be obtained to expand our knowledge of substrates. In this work, phase-contrast tomography (PCT) was used to gather information about the structure of a model lignocellulosic biomass (piassava fibers). The three-dimensional cellular organization of piassava fibers was characterized by PCT using synchrotron radiation. This technique enabled important physical features that describe the substrate piassava fibers to be visualized and quantified. The external surface area of a fiber and internal surface area of the pores in a fiber could be determined separately. More than 96% of the overall surface area available to enzymes was in the bulk substrate. The pore surface area and length exhibited a positive linear relationship, where the slope of this relationship depended on the plant tissue. We demonstrated that PCT is a powerful tool for the three-dimensional characterization of the cell wall features related to biomass recalcitrance. Original and relevant quantitative information about the structural features of the analyzed material were obtained. The data obtained by PCT can be used to improve processing routes to efficiently convert biomass feedstock into sugars.
dc.description8
dc.description40
dc.languageeng
dc.relationBiotechnology For Biofuels
dc.relationBiotechnol Biofuels
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBiomass
dc.subjectPhase-contrast Tomography
dc.subjectRecalcitrance
dc.subjectSurface Area
dc.subjectSynchrotron Radiation
dc.titleTowards A Deeper Understanding Of Structural Biomass Recalcitrance Using Phase-contrast Tomography.
dc.typeArtículos de revistas


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