Immobilization Of Papain On Chitin And Chitosan And Recycling Of Soluble Enzyme For Deflocculation Of Saccharomyces Cerevisiae From Bioethanol Distilleries.

dc.creatorSilva, Douglas Fernandes
dc.creatorRosa, Henrique
dc.creatorCarvalho, Ana Flavia Azevedo
dc.creatorOliva-Neto, Pedro
dc.date2015
dc.date2015-11-27T13:45:59Z
dc.date2015-11-27T13:45:59Z
dc.date.accessioned2018-03-29T01:23:22Z
dc.date.available2018-03-29T01:23:22Z
dc.identifierEnzyme Research. v. 2015, p. 573721, 2015.
dc.identifier2090-0406
dc.identifier10.1155/2015/573721
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25628895
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/202078
dc.identifier25628895
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1302311
dc.descriptionYeast flocculation (Saccharomyces cerevisiae) is one of the most important problems in fuel ethanol production. Yeast flocculation causes operational difficulties and increase in the ethanol cost. Proteolytic enzymes can solve this problem since it does not depend on these changes. The recycling of soluble papain and the immobilization of this enzyme on chitin or chitosan were studied. Some cross-linking agents were evaluated in the action of proteolytic activity of papain. The glutaraldehyde (0.1-10% w·v(-1)), polyethyleneimine (0.5% v·v(-1)), and tripolyphosphate (1-10% w·v(-1)) inactivated the enzyme in this range, respectively. Glutaraldehyde inhibited all treatments of papain immobilization. The chitosan cross-linked with TPP in 5 h of reaction showed the yield of active immobilized enzyme of 15.7% and 6.07% in chitosan treated with 0.1% PEI. Although these immobilizations have been possible, these levels have not been enough to cause deflocculation of yeast cells. Free enzyme was efficient for yeast deflocculation in dosages of 3 to 4 g·L(-1). Recycling of soluble papain by centrifugation was effective for 14 cycles with yeast suspension in time perfectly compatible to industrial conditions. The reuse of proteases applied after yeast suspension by additional yeast centrifugation could be an alternative to cost reduction of these enzymes.
dc.description2015
dc.description573721
dc.languageeng
dc.relationEnzyme Research
dc.relationEnzyme Res
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.titleImmobilization Of Papain On Chitin And Chitosan And Recycling Of Soluble Enzyme For Deflocculation Of Saccharomyces Cerevisiae From Bioethanol Distilleries.
dc.typeArtículos de revistas


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