Analysis Of Cellular Adhesion On Superhydrophobic And Superhydrophilic Vertically Aligned Carbon Nanotube Scaffolds.

dc.creatorMachado, M M
dc.creatorLobo, A O
dc.creatorMarciano, F R
dc.creatorCorat, E J
dc.creatorCorat, M A F
dc.date2015-Mar
dc.date2015-11-27T13:45:52Z
dc.date2015-11-27T13:45:52Z
dc.date.accessioned2018-03-29T01:23:11Z
dc.date.available2018-03-29T01:23:11Z
dc.identifierMaterials Science & Engineering. C, Materials For Biological Applications. v. 48, p. 365-71, 2015-Mar.
dc.identifier1873-0191
dc.identifier10.1016/j.msec.2014.11.062
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25579935
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/202032
dc.identifier25579935
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1302265
dc.descriptionWe analyzed GFP cells after 24h cultivated on superhydrophilic vertically aligned carbon nanotube scaffolds. We produced two different densities of VACNT scaffolds on Ti using Ni or Fe catalysts. A simple and fast oxygen plasma treatment promoted the superhydrophilicity of them. We used five different substrates, such as: as-grown VACNT produced using Ni as catalyst (Ni), as-grown VACNT produced using Fe as catalyst (Fe), VACNT-O produced using Ni as catalyst (NiO), VACNT-O produced using Fe as catalyst (FeO) and Ti (control). The 4',6-diamidino-2-phenylindole reagent nuclei stained the adherent cells cultivated on five different analyzed scaffolds. We used fluorescence microscopy for image collect, ImageJ® to count adhered cell and GraphPad Prism 5® for statistical analysis. We demonstrated in crescent order: Fe, Ni, NiO, FeO and Ti scaffolds that had an improved cellular adhesion. Oxygen treatment associated to high VACNT density (group FeO) presented significantly superior cell adhesion up to 24h. However, they do not show significant differences compared with Ti substrates (control). We demonstrated that all the analyzed substrates were nontoxic. Also, we proposed that the density and hydrophilicity influenced the cell adhesion behavior.
dc.description48
dc.description365-71
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.relationMaterials Science & Engineering. C, Materials For Biological Applications
dc.relationMater Sci Eng C Mater Biol Appl
dc.rightsfechado
dc.rightsCopyright © 2014 Elsevier B.V. All rights reserved.
dc.sourcePubMed
dc.subjectCarbon Nanotube
dc.subjectCell Adhesion
dc.subjectEmbryonic Fibroblasts
dc.subjectGreen Fluorescent Protein
dc.subjectSuperhydrophilicity
dc.subjectTransgenic Mice
dc.titleAnalysis Of Cellular Adhesion On Superhydrophobic And Superhydrophilic Vertically Aligned Carbon Nanotube Scaffolds.
dc.typeArtículos de revistas


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