Inhibition Of Tumor Necrosis Factor-alpha And Cyclooxigenase-2 By Isatin: A Molecular Mechanism Of Protection Against Tnbs-induced Colitis In Rats.

dc.creatorSocca, Eduardo Augusto Rabelo
dc.creatorLuiz-Ferreira, Anderson
dc.creatorde Faria, Felipe Meira
dc.creatorde Almeida, Ana Cristina
dc.creatorDunder, Ricardo José
dc.creatorManzo, Luis Paulo
dc.creatorBrito, Alba Regina Monteiro Souza
dc.date2014-Feb
dc.date2015-11-27T13:43:54Z
dc.date2015-11-27T13:43:54Z
dc.date.accessioned2018-03-29T01:22:48Z
dc.date.available2018-03-29T01:22:48Z
dc.identifierChemico-biological Interactions. v. 209, p. 48-55, 2014-Feb.
dc.identifier1872-7786
dc.identifier10.1016/j.cbi.2013.11.019
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24316276
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201925
dc.identifier24316276
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1302158
dc.descriptionIsatin, an indole alkaloid has been shown to have anti-microbial, anti-tumor and anti-inflammatory effects. Due to its findings, we evaluated whether this alkaloid would have any effect on TNBS-induced colitis. Animals (male Unib:WH rats, aged 8 weeks old) were induced colitis through a rectal administration of 2,4,6-trinitrobenzene sulphonic acid using a catheter inserted 8 cm into the rectum of the animals. The rats were divided into two major groups: non-colitic and colitic. The colitic group was sub-divided into 6 groups (10 animals per group): colitic non-treated, Isatin 3; 6; 12.5; 18.75 and 25 mg/kg. Our main results showed that the oral treatment with Isatin 6 and 25 mg/kg were capable of avoiding the increase in TNF-α, COX-2 and PGE₂ levels when compared to the colitic non-treated group. Interestingly, the same doses (6 and 25 mg/kg) were also capable of preventing the decrease in IL-10 levels comparing with the colitic non-treated group. The levels of MPO, (an indirect indicator of neutrophil presence), were also maintained lower than those of the colitic non-treated group. Isatin also prevented the decrease of SOD activity and increase of GSH-Px and GSH-Rd activity as well as the depletion of GSH levels. In conclusion, both pre-treatments (6 and 25 mg/kg) were capable of protecting the gut mucosa against the injury caused by TNBS, through the combination of antioxidant and anti-inflammatory properties, which, together, showed a protective activity of the indole alkaloid Isatin.
dc.description209
dc.description48-55
dc.languageeng
dc.relationChemico-biological Interactions
dc.relationChem. Biol. Interact.
dc.rightsfechado
dc.rightsCopyright © 2013 Elsevier Ireland Ltd. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBlotting, Western
dc.subjectColitis
dc.subjectCyclooxygenase 2
dc.subjectCyclooxygenase 2 Inhibitors
dc.subjectDisease Models, Animal
dc.subjectEnzyme Activation
dc.subjectGlutathione
dc.subjectIsatin
dc.subjectRats
dc.subjectTrinitrobenzenesulfonic Acid
dc.subjectTumor Necrosis Factor-alpha
dc.subjectCox-2
dc.subjectIsatin
dc.subjectMyeloperoxidase
dc.subjectTnbs
dc.subjectTnf-α
dc.subjectUlcerative Colitis
dc.titleInhibition Of Tumor Necrosis Factor-alpha And Cyclooxigenase-2 By Isatin: A Molecular Mechanism Of Protection Against Tnbs-induced Colitis In Rats.
dc.typeArtículos de revistas


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