Granulocyte Colony-stimulating Factor (g-csf) Positive Effects On Muscle Fiber Degeneration And Gait Recovery After Nerve Lesion In Mdx Mice.

dc.creatorSimões, Gustavo F
dc.creatorBenitez, Suzana U
dc.creatorOliveira, Alexandre L R
dc.date2014-Sep
dc.date2015-11-27T13:43:38Z
dc.date2015-11-27T13:43:38Z
dc.date.accessioned2018-03-29T01:22:21Z
dc.date.available2018-03-29T01:22:21Z
dc.identifierBrain And Behavior. v. 4, n. 5, p. 738-53, 2014-Sep.
dc.identifier2162-3279
dc.identifier10.1002/brb3.250
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25328849
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201814
dc.identifier25328849
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1302047
dc.descriptionG-CSF has been shown to decrease inflammatory processes and to act positively on the process of peripheral nerve regeneration during the course of muscular dystrophy. The aims of this study were to investigate the effects of treatment of G-CSF during sciatic nerve regeneration and histological analysis in the soleus muscle in MDX mice. Six-week-old male MDX mice underwent left sciatic nerve crush and were G-CSF treated at 7 days prior to and 21 days after crush. Ten and twenty-one days after surgery, the mice were euthanized, and the sciatic nerves were processed for immunohistochemistry (anti-p75(NTR) and anti-neurofilament) and transmission electron microscopy. The soleus muscles were dissected out and processed for H&E staining and subsequent morphologic analysis. Motor function analyses were performed at 7 days prior to and 21 days after sciatic crush using the CatWalk system and the sciatic nerve index. Both groups treated with G-CSF showed increased p75(NTR) and neurofilament expression after sciatic crush. G-CSF treatment decreased the number of degenerated and regenerated muscle fibers, thereby increasing the number of normal muscle fibers. The reduction in p75(NTR) and neurofilament indicates a decreased regenerative capacity in MDX mice following a lesion to a peripheral nerve. The reduction in motor function in the crushed group compared with the control groups may reflect the cycles of muscle degeneration/regeneration that occur postnatally. Thus, G-CSF treatment increases motor function in MDX mice. Nevertheless, the decrease in baseline motor function in these mice is not reversed completely by G-CSF.
dc.description4
dc.description738-53
dc.languageeng
dc.relationBrain And Behavior
dc.relationBrain Behav
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAxotomy
dc.subjectG-csf
dc.subjectSchwann Cell
dc.subjectMuscular Dystrophy
dc.titleGranulocyte Colony-stimulating Factor (g-csf) Positive Effects On Muscle Fiber Degeneration And Gait Recovery After Nerve Lesion In Mdx Mice.
dc.typeArtículos de revistas


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