Chemical Composition And Free Radical-scavenging, Anticancer And Anti-inflammatory Activities Of The Essential Oil From Ocimum Kilimandscharicum.

dc.creatorde Lima, V T
dc.creatorVieira, M C
dc.creatorKassuya, C A L
dc.creatorCardoso, C A L
dc.creatorAlves, J M
dc.creatorFoglio, M A
dc.creatorde Carvalho, J E
dc.creatorFormagio, A S N
dc.date2014-Sep
dc.date2015-11-27T13:43:15Z
dc.date2015-11-27T13:43:15Z
dc.date.accessioned2018-03-29T01:21:49Z
dc.date.available2018-03-29T01:21:49Z
dc.identifierPhytomedicine : International Journal Of Phytotherapy And Phytopharmacology. v. 21, n. 11, p. 1298-302, 2014-Sep.
dc.identifier1618-095X
dc.identifier10.1016/j.phymed.2014.07.004
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25172793
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201674
dc.identifier25172793
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301907
dc.descriptionThe essential oil from the leaves of Ocimum kilimandscharicum (EOOK), collected in Dourados-MS, was investigated for anticancer, anti-inflammatory and antioxidant activity and chemical composition. The essential oil was extracted by hydrodistillation, and the chemical composition was performed by gas chromatography-mass spectrometry. The essential oil was evaluated for free radical-scavenging activity using the DPPH assay and was tested in an anticancer assay against ten human cancer cell lines. The response parameter (GI50) was calculated for the cell lines tested. The anti-inflammatory activity was evaluated using carrageenan-induced pleurisy in mice. The chemical composition showed 45 components with a predominance of monoterpenes, such as camphor (51.81%), 1,8 cineole (20.13%) and limonene (11.23%). The EOOK exhibited potent free radical-scavenging activity by the DPPH assay with a GI50 of 8.31 μg/ml. The major constituents, pure camphor (IC50=12.56 μg/ml) and mixture of the limonene: 1, 8 cineole (IC50=23.25 μg/ml) displayed a potent activity. The oral administration of EOOK (at 30 and 100 mg kg(-1)), as well as the pure camphor or a mixture of 1,8 cineole with limonene, significantly inhibited the carrageenan (Cg) induced pleurisy, reducing the migration of total leukocytes in mice by 82 ± 4% (30 mg kg(-1) of EOOK), 95 ± 4% (100 mg kg(-1) of EOOK), 83 ± 9% (camphor) and 80 ± 5% (mixture of 1,8 cineole:limonene 1:1). In vitro cytotoxicity screening against a human ovarian cancer cell line displayed high selectivity and potent anticancer activity with GI50=31.90 mg ml(-1). This work describes the anti-inflammatory, anticancer and antioxidant effects of EOOK for the first time. The essential oil exhibited marked anti-inflammatory, antioxidant and anticancer effects, an effect that can be attributed the presence of majorital compounds, and the response profiles from chemical composition differed from other oils collected in different locales.
dc.description21
dc.description1298-302
dc.languageeng
dc.relationPhytomedicine : International Journal Of Phytotherapy And Phytopharmacology
dc.relationPhytomedicine
dc.rightsfechado
dc.rightsCopyright © 2014. Published by Elsevier GmbH.
dc.sourcePubMed
dc.subjectAnti-inflammation
dc.subjectAnticancer
dc.subjectAntioxidant Activity
dc.subjectCg–ms
dc.subjectLamiaceae
dc.subjectOcimum Kilimandscharicum
dc.titleChemical Composition And Free Radical-scavenging, Anticancer And Anti-inflammatory Activities Of The Essential Oil From Ocimum Kilimandscharicum.
dc.typeArtículos de revistas


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