Evidences Of Endocytosis Via Caveolae Following Blood-brain Barrier Breakdown By Phoneutria Nigriventer Spider Venom.

dc.creatorSoares, Edilene Siqueira
dc.creatorMendonça, Monique Culturato Padilha
dc.creatorIrazusta, Silvia Pierre
dc.creatorCoope, Andressa
dc.creatorStávale, Leila Miguel
dc.creatorda Cruz-Höfling, Maria Alice
dc.date2014-Sep
dc.date2015-11-27T13:42:56Z
dc.date2015-11-27T13:42:56Z
dc.date.accessioned2018-03-29T01:21:23Z
dc.date.available2018-03-29T01:21:23Z
dc.identifierToxicology Letters. v. 229, n. 3, p. 415-22, 2014-Sep.
dc.identifier1879-3169
dc.identifier10.1016/j.toxlet.2014.07.018
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/25046252
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201559
dc.identifier25046252
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301792
dc.descriptionSpider venoms contain neurotoxic peptides aimed at paralyzing prey or for defense against predators; that is why they represent valuable tools for studies in neuroscience field. The present study aimed at identifying the process of internalization that occurs during the increased trafficking of vesicles caused by Phoneutria nigriventer spider venom (PNV)-induced blood-brain barrier (BBB) breakdown. Herein, we found that caveolin-1α is up-regulated in the cerebellar capillaries and Purkinje neurons of PNV-administered P14 (neonate) and 8- to 10-week-old (adult) rats. The white matter and granular layers were regions where caveolin-1α showed major upregulation. The variable age played a role in this effect. Caveolin-1 is the central protein that controls caveolae formation. Caveolar-specialized cholesterol- and sphingolipid-rich membrane sub-domains are involved in endocytosis, transcytosis, mechano-sensing, synapse formation and stabilization, signal transduction, intercellular communication, apoptosis, and various signaling events, including those related to calcium handling. PNV is extremely rich in neurotoxic peptides that affect glutamate handling and interferes with ion channels physiology. We suggest that the PNV-induced BBB opening is associated with a high expression of caveolae frame-forming caveolin-1α, and therefore in the process of internalization and enhanced transcytosis. Caveolin-1α up-regulation in Purkinje neurons could be related to a way of neurons to preserve, restore, and enhance function following PNV-induced excitotoxicity. The findings disclose interesting perspectives for further molecular studies of the interaction between PNV and caveolar specialized membrane domains. It proves PNV to be excellent tool for studies of transcytosis, the most common form of BBB-enhanced permeability.
dc.description229
dc.description415-22
dc.languageeng
dc.relationToxicology Letters
dc.relationToxicol. Lett.
dc.rightsfechado
dc.rightsCopyright © 2014 Elsevier Ireland Ltd. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBlood-brain Barrier
dc.subjectBlotting, Western
dc.subjectBrain
dc.subjectCaveolae
dc.subjectCaveolin 1
dc.subjectEndocytosis
dc.subjectMale
dc.subjectNeuropeptides
dc.subjectNeurotoxins
dc.subjectPurkinje Cells
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReal-time Polymerase Chain Reaction
dc.subjectSpider Venoms
dc.subjectSpiders
dc.subjectBbb
dc.subjectCaveolin-1
dc.subjectEndocytosis
dc.subjectLipid Rafts
dc.subjectTranscellular Trafficking
dc.titleEvidences Of Endocytosis Via Caveolae Following Blood-brain Barrier Breakdown By Phoneutria Nigriventer Spider Venom.
dc.typeArtículos de revistas


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