[the Use Of Fish On Buccal Smear To Investigate Mosaicism With A 45,x Cell Line: Study On Healthy Men And Patients With Disorders Of Sex Development].

dc.creatorde Paulo, Juliana
dc.creatorde Andrade, Juliana Gabriel Ribeiro
dc.creatordos Santos, Ana Paula
dc.creatorGil-da-Silva-Lopes, Vera Lúcia
dc.creatorGuerra-Júnior, Gil
dc.creatorMaciel-Guerra, Andréa Trevas
dc.date2014-Jun
dc.date2015-11-27T13:42:45Z
dc.date2015-11-27T13:42:45Z
dc.date.accessioned2018-03-29T01:21:05Z
dc.date.available2018-03-29T01:21:05Z
dc.identifierArquivos Brasileiros De Endocrinologia E Metabologia. v. 58, n. 4, p. 328-34, 2014-Jun.
dc.identifier1677-9487
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24936726
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201483
dc.identifier24936726
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301716
dc.descriptionTo verify whether fluorescence in situ hybridization (FISH) of cells from the buccal epithelium could be employed to detect cryptomosaicism with a 45,X lineage in 46,XY patients. Samples of nineteen 46,XY healthy young men and five patients with disorders of sex development (DSD), four 45,X/46,XY and one 46,XY were used. FISH analysis with X and Y specific probes on interphase nuclei from blood lymphocytes and buccal epithelium were analyzed to investigate the proportion of nuclei containing only the signal of the X chromosome. The frequency of nuclei containing only the X signal in the two tissues of healthy men did not differ (p = 0.69). In all patients with DSD this frequency was significantly higher, and there was no difference between the two tissues (p = 0.38), either. Investigation of mosaicism with a 45,X cell line in patients with 46,XY DSD or sterility can be done by FISH directly using cells from the buccal epithelium.
dc.description58
dc.description328-34
dc.languagepor
dc.relationArquivos Brasileiros De Endocrinologia E Metabologia
dc.relationArq Bras Endocrinol Metabol
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.title[the Use Of Fish On Buccal Smear To Investigate Mosaicism With A 45,x Cell Line: Study On Healthy Men And Patients With Disorders Of Sex Development].
dc.typeArtículos de revistas


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