Comparison Of The Solution Of Histidine-tryptophan-alfacetoglutarate With Histidine-tryptophan-glutamate As Cardioplegic Agents In Isolated Rat Hearts: An Immunohistochemical Study.

dc.creatorOliveira, Marcos Aurélio Barboza de
dc.creatorFerreira, Lívia Carvalho
dc.creatorZuccari, Débora Aparecida Pires de Campos
dc.creatorBrandi, Antônio Carlos
dc.creatorSantos, Carlos Alberto dos
dc.creatorBotelho, Paulo Henrique Husseni
dc.creatorPetrucci, Orlando
dc.creatorBraile, Domingo Marcolino
dc.date
dc.date2015-11-27T13:42:40Z
dc.date2015-11-27T13:42:40Z
dc.date.accessioned2018-03-29T01:20:58Z
dc.date.available2018-03-29T01:20:58Z
dc.identifierRevista Brasileira De Cirurgia Cardiovascular : órgão Oficial Da Sociedade Brasileira De Cirurgia Cardiovascular. v. 29, n. 1, p. 83-8
dc.identifier1678-9741
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24896167
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201453
dc.identifier24896167
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301686
dc.descriptionCardiac arrest during heart surgery is a common procedure and allows the surgeon to perform surgical procedures in an environment free of blood and movement. Using a model of isolated rat heart, the authors compare a new cardioplegic solution containing histidine-tryptophan-glutamate (group 2) with the histidine-tryptophan-alphacetoglutarate (group 1) routinely used by some cardiac surgeons. To assess caspase, IL-8 and KI-67 in isolated rat hearts using immunohistochemistry. 20 Wistar male rats were anesthetized and heparinized. The chest was opened, cardioctomy was performed and 40 ml/kg of the appropriate cardioplegic solution was infused. The hearts were kept for 2 hours at 4ºC in the same solution, and thereafter, placed in the Langendorff apparatus for 30 minutes with Ringer-Locke solution. Immunohistochemistry analysis of caspase, IL-8, and KI-67 were performed. The concentration of caspase was lower in group 2 and Ki-67 was higher in group 2, both P<0.05. There was no statistical difference between the values of IL-8 between the groups. Histidine-tryptophan-glutamate solution was better than histidine-tryptophan-alphacetoglutarate solution because it reduced caspase (apoptosis), increased KI-67 (cell proliferation), and showed no difference in IL-8 levels compared to group 1. This suggests that the histidine-tryptophan-glutamate solution was more efficient than the histidine-tryptophan-alphacetoglutarate for the preservation of hearts of rat cardiomyocytes.
dc.description29
dc.description83-8
dc.languageeng
dc.languagepor
dc.relationRevista Brasileira De Cirurgia Cardiovascular : órgão Oficial Da Sociedade Brasileira De Cirurgia Cardiovascular
dc.relationRev Bras Cir Cardiovasc
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectCardioplegic Solutions
dc.subjectCaspases
dc.subjectGlutamic Acid
dc.subjectGlutarates
dc.subjectHeart
dc.subjectHistidine
dc.subjectImmunohistochemistry
dc.subjectInterleukin-8
dc.subjectKi-67 Antigen
dc.subjectMale
dc.subjectMyocytes, Cardiac
dc.subjectRats, Wistar
dc.subjectReproducibility Of Results
dc.subjectTime Factors
dc.subjectTryptophan
dc.titleComparison Of The Solution Of Histidine-tryptophan-alfacetoglutarate With Histidine-tryptophan-glutamate As Cardioplegic Agents In Isolated Rat Hearts: An Immunohistochemical Study.
dc.typeArtículos de revistas


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