Antioxidant And Antiproliferative Activities Of Methanolic Extract From A Neglected Agricultural Product: Corn Cobs.

dc.creatorMelo-Silveira, Raniere Fagundes
dc.creatorFidelis, Gabriel Pereira
dc.creatorViana, Rony Lucas Silva
dc.creatorSoeiro, Vinícius Campelo
dc.creatorSilva, Rodrigo Augusto da
dc.creatorMachado, Daisy
dc.creatorCosta, Leandro Silva
dc.creatorFerreira, Carmen Veríssima
dc.creatorOliveira Rocha, Hugo Alexandre
dc.date2014
dc.date2015-11-27T13:42:39Z
dc.date2015-11-27T13:42:39Z
dc.date.accessioned2018-03-29T01:20:56Z
dc.date.available2018-03-29T01:20:56Z
dc.identifierMolecules (basel, Switzerland). v. 19, n. 4, p. 5360-78, 2014.
dc.identifier1420-3049
dc.identifier10.3390/molecules19045360
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24879583
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201442
dc.identifier24879583
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301675
dc.descriptionNeglected agricultural products (NAPs) are defined as discarded material in agricultural production. Corn cobs are a major waste of agriculture maize. Here, a methanolic extract from corn cobs (MEC) was obtained. MEC contains phenolic compounds, protein, carbohydrates (1.4:0.001:0.001). We evaluated the in vitro and in vivo antioxidant potential of MEC. Furthermore, its antiproliferative property against tumor cells was assessed through MTT assays and proteins related to apoptosis in tumor cells were examined by western blot. MEC showed no hydroxyl radical scavenger capacity, but it showed antioxidant activity in Total Antioxidant Capacity and DPPH scavenger ability assays. MEC showed higher Reducing Power than ascorbic acid and exhibited high Superoxide Scavenging activity. In tumor cell culture, MEC increased catalase, metallothionein and superoxide dismutase expression in accordance with the antioxidant tests. In vivo antioxidant test, MEC restored SOD and CAT, decreased malondialdehyde activities and showed high Trolox Equivalent Antioxidant Capacity in animals treated with CCl4. Furthermore, MEC decreased HeLa cells viability by apoptosis due an increase of Bax/Bcl-2 ratio, caspase 3 active. Protein kinase C expression increased was also detected in treated tumor cells. Thus, our findings pointed out the biotechnological potential of corn cobs as a source of molecules with pharmacological activity.
dc.description19
dc.description5360-78
dc.languageeng
dc.relationMolecules (basel, Switzerland)
dc.relationMolecules
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAntineoplastic Agents, Phytogenic
dc.subjectApoptosis
dc.subjectCatalase
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectDrug Screening Assays, Antitumor
dc.subjectFree Radical Scavengers
dc.subjectHela Cells
dc.subjectHumans
dc.subjectInhibitory Concentration 50
dc.subjectMetallothionein
dc.subjectMethanol
dc.subjectOxidation-reduction
dc.subjectPlant Components, Aerial
dc.subjectPlant Extracts
dc.subjectRats, Wistar
dc.subjectSolid Phase Extraction
dc.subjectSolvents
dc.subjectSuperoxide Dismutase
dc.subjectSuperoxides
dc.subjectZea Mays
dc.titleAntioxidant And Antiproliferative Activities Of Methanolic Extract From A Neglected Agricultural Product: Corn Cobs.
dc.typeArtículos de revistas


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