Neutralisation Of The Pharmacological Activities Of Bothrops Alternatus Venom By Anti-pla2 Iggs.

dc.creatorGarcia Denegri, María E
dc.creatorMaruñak, Silvana
dc.creatorTodaro, Juan S
dc.creatorPonce-Soto, Luis A
dc.creatorAcosta, Ofelia
dc.creatorLeiva, Laura
dc.date2014-Aug
dc.date2015-11-27T13:42:38Z
dc.date2015-11-27T13:42:38Z
dc.date.accessioned2018-03-29T01:20:55Z
dc.date.available2018-03-29T01:20:55Z
dc.identifierToxicon : Official Journal Of The International Society On Toxinology. v. 86, p. 89-95, 2014-Aug.
dc.identifier1879-3150
dc.identifier10.1016/j.toxicon.2014.05.016
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24878372
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201439
dc.identifier24878372
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301672
dc.descriptionBasic phospholipases A2 (PLA2) are toxic and induce a wide spectrum of pharmacological effects, although the acidic enzyme types are not lethal or cause low lethality. Therefore, it is challenging to elucidate the mechanism of action of acidic phospholipases. This study used the acidic non-toxic Ba SpII RP4 PLA2 from Bothrops alternatus as an antigen to develop anti-PLA2 IgG antibodies in rabbits and used in vivo assays to examine the changes in crude venom when pre-incubated with these antibodies. Using Ouchterlony and western blot analyses on B. alternatus venom, we examined the specificity and sensitivity of phospholipase A2 recognition by the specific antibodies (anti-PLA2 IgG). Neutralisation assays using a non-toxic PLA2 antigen revealed unexpected results. The (indirect) haemolytic activity of whole venom was completely inhibited, and all catalytically active phospholipases A2 were blocked. Myotoxicity and lethality were reduced when the crude venom was pre-incubated with anti-PLA2 immunoglobulins. CK levels in the skeletal muscle were significantly reduced at 6 h, and the muscular damage was more significant at this time-point compared to 3 and 12 h. When four times the LD50 was used (224 μg), half the animals treated with the venom-anti PLA2 IgG mixture survived after 48 h. All assays performed with the specific antibodies revealed that Ba SpII RP4 PLA2 had a synergistic effect on whole-venom toxicity. IgG antibodies against the venom of the Argentinean species B. alternatus represent a valuable tool for elucidation of the roles of acidic PLA2 that appear to have purely digestive roles and for further studies on immunotherapy and snake envenoming in affected areas in Argentina and Brazil.
dc.description86
dc.description89-95
dc.languageeng
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.relationToxicon
dc.rightsfechado
dc.rightsCopyright © 2014 Elsevier Ltd. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAntibodies, Anti-idiotypic
dc.subjectAntibodies, Neutralizing
dc.subjectAntibody Specificity
dc.subjectAntivenins
dc.subjectBothrops
dc.subjectCrotalid Venoms
dc.subjectImmunoglobulin G
dc.subjectMale
dc.subjectMice
dc.subjectNeutralization Tests
dc.subjectPhospholipase A2 Inhibitors
dc.subjectPhospholipases A2
dc.subjectRabbits
dc.subjectAnti-pla(2) Immunoglobulins
dc.subjectBothrops Alternatus
dc.subjectEnvenomation
dc.subjectNon-myotoxic Pla(2)
dc.titleNeutralisation Of The Pharmacological Activities Of Bothrops Alternatus Venom By Anti-pla2 Iggs.
dc.typeArtículos de revistas


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