Carbon Nanoparticles For Gene Transfection In Eukaryotic Cell Lines.

dc.creatorZanin, H
dc.creatorHollanda, L M
dc.creatorCeragioli, H J
dc.creatorFerreira, M S
dc.creatorMachado, D
dc.creatorLancellotti, M
dc.creatorCatharino, R R
dc.creatorBaranauskas, V
dc.creatorLobo, A O
dc.date2014-Jun
dc.date2015-11-27T13:42:36Z
dc.date2015-11-27T13:42:36Z
dc.date.accessioned2018-03-29T01:20:53Z
dc.date.available2018-03-29T01:20:53Z
dc.identifierMaterials Science & Engineering. C, Materials For Biological Applications. v. 39, p. 359-70, 2014-Jun.
dc.identifier1873-0191
dc.identifier10.1016/j.msec.2014.03.016
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24863237
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201428
dc.identifier24863237
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301661
dc.descriptionFor the first time, oxygen terminated cellulose carbon nanoparticles (CCN) was synthesised and applied in gene transfection of pIRES plasmid. The CCN was prepared from catalytic of polyaniline by chemical vapour deposition techniques. This plasmid contains one gene that encodes the green fluorescent protein (GFP) in eukaryotic cells, making them fluorescent. This new nanomaterial and pIRES plasmid formed π-stacking when dispersed in water by magnetic stirring. The frequencies shift in zeta potential confirmed the plasmid strongly connects to the nanomaterial. In vitro tests found that this conjugation was phagocytised by NG97, NIH-3T3 and A549 cell lines making them fluorescent, which was visualised by fluorescent microscopy. Before the transfection test, we studied CCN in cell viability. Both MTT and Neutral Red uptake tests were carried out using NG97, NIH-3T3 and A549 cell lines. Further, we use metabolomics to verify if small amounts of nanomaterial would be enough to cause some cellular damage in NG97 cells. We showed two mechanisms of action by CCN-DNA complex, producing an exogenous protein by the transfected cell and metabolomic changes that contributed by better understanding of glioblastoma, being the major finding of this work. Our results suggested that this nanomaterial has great potential as a gene carrier agent in non-viral based therapy, with low cytotoxicity, good transfection efficiency, and low cell damage in small amounts of nanomaterials in metabolomic tests.
dc.description39
dc.description359-70
dc.languageeng
dc.relationMaterials Science & Engineering. C, Materials For Biological Applications
dc.relationMater Sci Eng C Mater Biol Appl
dc.rightsfechado
dc.rightsCopyright © 2014 Elsevier B.V. All rights reserved.
dc.sourcePubMed
dc.subject3t3 Cells
dc.subjectAnimals
dc.subjectCarbon
dc.subjectCell Line, Tumor
dc.subjectCell Survival
dc.subjectCellulose
dc.subjectDna
dc.subjectGreen Fluorescent Proteins
dc.subjectHumans
dc.subjectMetabolomics
dc.subjectMice
dc.subjectMicroscopy, Electron, Scanning
dc.subjectNanoparticles
dc.subjectParticle Size
dc.subjectPlasmids
dc.subjectSpectroscopy, Fourier Transform Infrared
dc.subjectSpectrum Analysis, Raman
dc.subjectTransfection
dc.subjectCarbon Nanomaterial
dc.subjectCell Viability
dc.subjectGene Transfection
dc.subjectMetabolomic Test
dc.titleCarbon Nanoparticles For Gene Transfection In Eukaryotic Cell Lines.
dc.typeArtículos de revistas


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