Solid Lipid Nanoparticles For Hydrophilic Biotech Drugs: Optimization And Cell Viability Studies (caco-2 & Hepg-2 Cell Lines).

dc.creatorSeverino, Patrícia
dc.creatorAndreani, Tatiana
dc.creatorJäger, Alessandro
dc.creatorChaud, Marco V
dc.creatorSantana, Maria Helena A
dc.creatorSilva, Amélia M
dc.creatorSouto, Eliana B
dc.date2014-Jun
dc.date2015-11-27T13:42:31Z
dc.date2015-11-27T13:42:31Z
dc.date.accessioned2018-03-29T01:20:45Z
dc.date.available2018-03-29T01:20:45Z
dc.identifierEuropean Journal Of Medicinal Chemistry. v. 81, p. 28-34, 2014-Jun.
dc.identifier1768-3254
dc.identifier10.1016/j.ejmech.2014.04.084
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24819957
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201393
dc.identifier24819957
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301626
dc.descriptionInsulin was used as model protein to developed innovative Solid Lipid Nanoparticles (SLNs) for the delivery of hydrophilic biotech drugs, with potential use in medicinal chemistry. SLNs were prepared by double emulsion with the purpose of promoting stability and enhancing the protein bioavailability. Softisan(®)100 was selected as solid lipid matrix. The surfactants (Tween(®)80, Span(®)80 and Lipoid(®)S75) and insulin were chosen applying a 2(2) factorial design with triplicate of central point, evaluating the influence of dependents variables as polydispersity index (PI), mean particle size (z-AVE), zeta potential (ZP) and encapsulation efficiency (EE) by factorial design using the ANOVA test. Therefore, thermodynamic stability, polymorphism and matrix crystallinity were checked by Differential Scanning Calorimetry (DSC) and Wide Angle X-ray Diffraction (WAXD), whereas the effect of toxicity of SLNs was check in HepG2 and Caco-2 cells. Results showed a mean particle size (z-AVE) width between 294.6 nm and 627.0 nm, a PI in the range of 0.425-0.750, ZP about -3 mV, and the EE between 38.39% and 81.20%. After tempering the bulk lipid (mimicking the end process of production), the lipid showed amorphous characteristics, with a melting point of ca. 30 °C. The toxicity of SLNs was evaluated in two distinct cell lines (HEPG-2 and Caco-2), showing to be dependent on the concentration of particles in HEPG-2 cells, while no toxicity in was reported in Caco-2 cells. SLNs were stable for 24 h in in vitro human serum albumin (HSA) solution. The resulting SLNs fabricated by double emulsion may provide a promising approach for administration of protein therapeutics and antigens.
dc.description81
dc.description28-34
dc.languageeng
dc.relationEuropean Journal Of Medicinal Chemistry
dc.relationEur J Med Chem
dc.rightsfechado
dc.rightsCopyright © 2014 Elsevier Masson SAS. All rights reserved.
dc.sourcePubMed
dc.subjectCaco-2 Cell Lines
dc.subjectDouble Emulsion
dc.subjectHepg-2 Cell Lines
dc.subjectHydrophilic Biotech Drugs
dc.subjectLipid Nanoparticles
dc.subjectInsulin
dc.titleSolid Lipid Nanoparticles For Hydrophilic Biotech Drugs: Optimization And Cell Viability Studies (caco-2 & Hepg-2 Cell Lines).
dc.typeArtículos de revistas


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