An Asp49 Phospholipase A2 From Snake Venom Induces Cyclooxygenase-2 Expression And Prostaglandin E2 Production Via Activation Of Nf-κb, P38mapk, And Pkc In Macrophages.

dc.creatorMoreira, Vanessa
dc.creatorLomonte, Bruno
dc.creatorVinolo, Marco Aurélio Ramirez
dc.creatorCuri, Rui
dc.creatorGutiérrez, José María
dc.creatorTeixeira, Catarina
dc.date2014
dc.date2015-11-27T13:42:29Z
dc.date2015-11-27T13:42:29Z
dc.date.accessioned2018-03-29T01:20:42Z
dc.date.available2018-03-29T01:20:42Z
dc.identifierMediators Of Inflammation. v. 2014, p. 105879, 2014.
dc.identifier1466-1861
dc.identifier10.1155/2014/105879
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24808633
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201384
dc.identifier24808633
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301617
dc.descriptionPhospholipases A2 (PLA2) are key enzymes for production of lipid mediators. We previously demonstrated that a snake venom sPLA2 named MT-III leads to prostaglandin (PG)E2 biosynthesis in macrophages by inducing the expression of cyclooxygenase-2 (COX-2). Herein, we explored the molecular mechanisms and signaling pathways leading to these MT-III-induced effects. Results demonstrated that MT-III induced activation of the transcription factor NF-κB in isolated macrophages. By using NF-κB selective inhibitors, the involvement of this factor in MT-III-induced COX-2 expression and PGE2 production was demonstrated. Moreover, MT-III-induced COX-2 protein expression and PGE2 release were attenuated by pretreatment of macrophages with SB202190, and Ly294002, and H-7-dihydro compounds, indicating the involvement of p38MAPK, PI3K, and PKC pathways, respectively. Consistent with this, MT-III triggered early phosphorylation of p38MAPK, PI3K, and PKC. Furthermore, SB202190, H-7-dihydro, but not Ly294002 treatment, abrogated activation of NF-κB induced by MT-III. Altogether, these results show for the first time that the induction of COX-2 protein expression and PGE2 release, which occur via NF-κB activation induced by the sPLA2-MT-III in macrophages, are modulated by p38MAPK and PKC, but not by PI3K signaling proteins.
dc.description2014
dc.description105879
dc.languageeng
dc.relationMediators Of Inflammation
dc.relationMediators Inflamm.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCells, Cultured
dc.subjectChromones
dc.subjectCyclooxygenase 2
dc.subjectDinoprostone
dc.subjectImidazoles
dc.subjectMacrophages
dc.subjectMale
dc.subjectMice
dc.subjectMorpholines
dc.subjectNf-kappa B
dc.subjectPhospholipases A2
dc.subjectProtein Kinase C
dc.subjectPyridines
dc.subjectSnake Venoms
dc.subjectP38 Mitogen-activated Protein Kinases
dc.titleAn Asp49 Phospholipase A2 From Snake Venom Induces Cyclooxygenase-2 Expression And Prostaglandin E2 Production Via Activation Of Nf-κb, P38mapk, And Pkc In Macrophages.
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución