Dna Extraction From Filter-paper Spots Of Vaginal Samples Collected After Sexual Violence.

dc.creatorCampos, Elisabete A
dc.creatorPitta, Denise R
dc.creatorCosta, Fernanda A
dc.creatorCampos, Vanessa M
dc.creatorYela, Daniela
dc.creatorFernandes, Arlete
dc.date2014-Jul
dc.date2015-11-27T13:42:28Z
dc.date2015-11-27T13:42:28Z
dc.date.accessioned2018-03-29T01:20:39Z
dc.date.available2018-03-29T01:20:39Z
dc.identifierInternational Journal Of Gynaecology And Obstetrics: The Official Organ Of The International Federation Of Gynaecology And Obstetrics. v. 126, n. 1, p. 23-7, 2014-Jul.
dc.identifier1879-3479
dc.identifier10.1016/j.ijgo.2014.02.010
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24792405
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201371
dc.identifier24792405
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301604
dc.descriptionTo detect the presence of male DNA in vaginal samples collected from survivors of sexual violence and stored on filter paper. A pilot study was conducted to evaluate 10 vaginal samples spotted on sterile filter paper: 6 collected at random in April 2009 and 4 in October 2010. Time between sexual assault and sample collection was 4-48hours. After drying at room temperature, the samples were placed in a sterile envelope and stored for 2-3years until processing. DNA extraction was confirmed by polymerase chain reaction for human β-globin, and the presence of prostate-specific antigen (PSA) was quantified. The presence of the Y chromosome was detected using primers for sequences in the TSPY (Y7/Y8 and DYS14) and SRY genes. β-Globin was detected in all 10 samples, while 2 samples were positive for PSA. Half of the samples amplified the Y7/Y8 and DYS14 sequences of the TSPY gene and 30% amplified the SRY gene sequence of the Y chromosome. Four male samples and 1 female sample served as controls. Filter-paper spots stored for periods of up to 3years proved adequate for preserving genetic material from vaginal samples collected following sexual violence.
dc.description126
dc.description23-7
dc.languageeng
dc.relationInternational Journal Of Gynaecology And Obstetrics: The Official Organ Of The International Federation Of Gynaecology And Obstetrics
dc.relationInt J Gynaecol Obstet
dc.rightsfechado
dc.rightsCopyright © 2014 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.
dc.sourcePubMed
dc.subjectAdolescent
dc.subjectAdult
dc.subjectChromosomes, Human, Y
dc.subjectDna
dc.subjectFemale
dc.subjectHumans
dc.subjectKallikreins
dc.subjectMale
dc.subjectPilot Projects
dc.subjectProstate-specific Antigen
dc.subjectSex Offenses
dc.subjectSpecimen Handling
dc.subjectTime Factors
dc.subjectVagina
dc.subjectYoung Adult
dc.subjectDna
dc.subjectFilter Paper
dc.subjectSexual Violence
dc.titleDna Extraction From Filter-paper Spots Of Vaginal Samples Collected After Sexual Violence.
dc.typeArtículos de revistas


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