Flower Development In Coffea Arabica L.: New Insights Into Mads-box Genes.

dc.creatorde Oliveira, Raphael Ricon
dc.creatorCesarino, Igor
dc.creatorMazzafera, Paulo
dc.creatorDornelas, Marcelo Carnier
dc.date2014-Jun
dc.date2015-11-27T13:42:17Z
dc.date2015-11-27T13:42:17Z
dc.date.accessioned2018-03-29T01:20:22Z
dc.date.available2018-03-29T01:20:22Z
dc.identifierPlant Reproduction. v. 27, n. 2, p. 79-94, 2014-Jun.
dc.identifier2194-7961
dc.identifier10.1007/s00497-014-0242-2
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24715004
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201299
dc.identifier24715004
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301532
dc.descriptionCoffea arabica L. shows peculiar characteristics during reproductive development, such as flowering asynchrony, periods of floral bud dormancy, mucilage secretion and epipetalous stamens. The MADS-box transcription factors are known to control several developmental processes in plants, including flower and fruit development. Significant differences are found among plant species regarding reproductive development and little is known about the role of MADS-box genes in Coffea reproductive development. Thus, we used anatomical and comparative molecular analyses to explore the flowering process in coffee. The main morphological changes during flower development in coffee were observed by optical and scanning electron microscopy. Flowering asynchrony seems to be related to two independent processes: the asynchronous development of distinct buds before the reproductive induction and the asynchronous development of floral meristems within each bud after the reproductive induction. A total of 23 C. arabica MADS-box genes were characterized by sequence comparison with putative Arabidopsis orthologs and their expression profiles were analyzed by RT-PCR in different tissues. The expression of the ABC model orthologs in Coffea during floral development was determined by in situ hybridization. The APETALA1 (AP1) ortholog is expressed only late in the perianth, which is also observed for the APETALA3 and TM6 orthologs. Conversely, the PISTILLATA ortholog is widely expressed in early stages, but restrict to stamens and carpels in later stages of flower development, while the expression of the AGAMOUS ortholog is always restricted to fertile organs. The AP1 and PISTILLATA orthologs are also expressed at specific floral organs, such as bracts and colleters, respectively, suggesting a potential role in the development of such structures. Altogether, the results from our comprehensive expression analyses showed significant differences between the spatiotemporal expression profiles of C. arabica MADS-box genes and their orthologs, which suggests differential functionalization in coffee. Moreover, these differences might also partially explain the particular characteristics of floral development in coffee, such as mucilage secretion and formation of epipetalous stamens.
dc.description27
dc.description79-94
dc.languageeng
dc.relationPlant Reproduction
dc.relationPlant Reprod
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectCoffea
dc.subjectFlowers
dc.subjectGene Expression Regulation, Developmental
dc.subjectGene Expression Regulation, Plant
dc.subjectMads Domain Proteins
dc.subjectMolecular Sequence Data
dc.subjectMultigene Family
dc.subjectPhylogeny
dc.subjectPlant Proteins
dc.titleFlower Development In Coffea Arabica L.: New Insights Into Mads-box Genes.
dc.typeArtículos de revistas


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