Behaviour Of The Oxidant Scavenger Metallothionein In Hypoxia-induced Neotropical Fish.

dc.creatorHauser-Davis, Rachel Ann
dc.creatorBastos, Frederico Freire
dc.creatorDantas, Rafael Ferreira
dc.creatorTobar, Santiago Alonso Leitão
dc.creatorda Cunha Bastos Neto, Jayme
dc.creatorda Cunha Bastos, Vera Lucia Freire
dc.creatorZiolli, Roberta Lourenço
dc.creatorArruda, Marco Aurélio Zezzi
dc.date2014-May
dc.date2015-11-27T13:41:58Z
dc.date2015-11-27T13:41:58Z
dc.date.accessioned2018-03-29T01:19:51Z
dc.date.available2018-03-29T01:19:51Z
dc.identifierEcotoxicology And Environmental Safety. v. 103, p. 24-8, 2014-May.
dc.identifier1090-2414
dc.identifier10.1016/j.ecoenv.2014.01.015
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24561243
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201165
dc.identifier24561243
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301398
dc.descriptionThe pacu (Piaractus mesopotamicus) is a hypoxia-tolerant neotropical fish species. There is little or no information in this species regarding biochemical adaptations to waters with different oxygen concentrations, such as the production of reactive oxygen species and antioxidant scavengers, which might be of interest in the study of antioxidant defense mechanisms. Metallothioneins (MT) have been widely applied as biomarkers for metal exposure in fish liver, and, recently, in bile. These metalloproteins, however, have also been reported as free radical scavengers, although studies in this regard are scarce in fish. In this context, normoxic and hypoxic controlled experiments were conducted with pacu specimens and MT levels were quantified in both liver and bile. Reduced glutathione (GSH) indicative of oxidative stress, and thiobarbituric acid reactive substances (TBARS), indicative of lipid peroxidation, were also determined in liver. The results demonstrate that hypoxic fish present significantly lower metallothionein levels in liver and bile and lower reduced glutathione levels in liver, whereas lipid peroxidation was not significantly different between hypoxic and normoxic fish. The results of the present study seem to suggest that metallothioneins may actively participate in redox regulation in hypoxic fish in both bile and liver. MT levels in these organs may be temporarily suppressed, supporting the notion that down-regulation of oxidant scavengers during the oxidative burst is important in defense signaling in these adapted organisms.
dc.description103
dc.description24-8
dc.languageeng
dc.relationEcotoxicology And Environmental Safety
dc.relationEcotoxicol. Environ. Saf.
dc.rightsfechado
dc.rightsCopyright © 2014 Elsevier Inc. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAnoxia
dc.subjectBile
dc.subjectCharaciformes
dc.subjectGlutathione
dc.subjectLipid Peroxidation
dc.subjectLiver
dc.subjectMetallothionein
dc.subjectOxidation-reduction
dc.subjectOxidative Stress
dc.subjectSpectrophotometry
dc.subjectThiobarbituric Acid Reactive Substances
dc.subjectFish Bile And Liver
dc.subjectGsh
dc.subjectHypoxia
dc.subjectMetallothionein
dc.subjectTbars
dc.titleBehaviour Of The Oxidant Scavenger Metallothionein In Hypoxia-induced Neotropical Fish.
dc.typeArtículos de revistas


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