Vegf Receptor Expression Decreases During Lung Development In Congenital Diaphragmatic Hernia Induced By Nitrofen.

dc.creatorSbragia, L
dc.creatorNassr, A C C
dc.creatorGonçalves, F L L
dc.creatorSchmidt, A F
dc.creatorZuliani, C C
dc.creatorGarcia, P V
dc.creatorGallindo, R M
dc.creatorPereira, L A V
dc.date2014-Feb
dc.date2015-11-27T13:41:54Z
dc.date2015-11-27T13:41:54Z
dc.date.accessioned2018-03-29T01:19:45Z
dc.date.available2018-03-29T01:19:45Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 47, n. 2, p. 171-8, 2014-Feb.
dc.identifier1414-431X
dc.identifier10.1590/1414-431X20133221
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24519134
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201136
dc.identifier24519134
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301369
dc.descriptionChanges in vascular endothelial growth factor (VEGF) in pulmonary vessels have been described in congenital diaphragmatic hernia (CDH) and may contribute to the development of pulmonary hypoplasia and hypertension; however, how the expression of VEGF receptors changes during fetal lung development in CDH is not understood. The aim of this study was to compare morphological evolution with expression of VEGF receptors, VEGFR1 (Flt-1) and VEGFR2 (Flk-1), in pseudoglandular, canalicular, and saccular stages of lung development in normal rat fetuses and in fetuses with CDH. Pregnant rats were divided into four groups (n=20 fetuses each) of four different gestational days (GD) 18.5, 19.5, 20.5, 21.5: external control (EC), exposed to olive oil (OO), exposed to 100 mg nitrofen, by gavage, without CDH (N-), and exposed to nitrofen with CDH (CDH) on GD 9.5 (term=22 days). The morphological variables studied were: body weight (BW), total lung weight (TLW), left lung weight, TLW/BW ratio, total lung volume, and left lung volume. The histometric variables studied were: left lung parenchymal area density and left lung parenchymal volume. VEGFR1 and VEGFR2 expression were determined by Western blotting. The data were analyzed using analysis of variance with the Tukey-Kramer post hoc test. CDH frequency was 37% (80/216). All the morphological and histometric variables were reduced in the N- and CDH groups compared with the controls, and reductions were more pronounced in the CDH group (P<0.05) and more evident on GD 20.5 and GD 21.5. Similar results were observed for VEGFR1 and VEGFR2 expression. We conclude that N- and CDH fetuses showed primary pulmonary hypoplasia, with a decrease in VEGFR1 and VEGFR2 expression.
dc.description47
dc.description171-8
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectDisease Models, Animal
dc.subjectFemale
dc.subjectHernias, Diaphragmatic, Congenital
dc.subjectLung
dc.subjectPhenyl Ethers
dc.subjectPregnancy
dc.subjectRats, Sprague-dawley
dc.subjectReceptors, Vascular Endothelial Growth Factor
dc.titleVegf Receptor Expression Decreases During Lung Development In Congenital Diaphragmatic Hernia Induced By Nitrofen.
dc.typeArtículos de revistas


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