The Gastroprotective Effect Of Menthol: Involvement Of Anti-apoptotic, Antioxidant And Anti-inflammatory Activities.

dc.creatorRozza, Ariane Leite
dc.creatorMeira de Faria, Felipe
dc.creatorSouza Brito, Alba Regina
dc.creatorPellizzon, Cláudia Helena
dc.date2014
dc.date2015-11-27T13:41:49Z
dc.date2015-11-27T13:41:49Z
dc.date.accessioned2018-03-29T01:19:37Z
dc.date.available2018-03-29T01:19:37Z
dc.identifierPlos One. v. 9, n. 1, p. e86686, 2014.
dc.identifier1932-6203
dc.identifier10.1371/journal.pone.0086686
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24466200
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201108
dc.identifier24466200
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301341
dc.descriptionThe aim of this research was to investigate the anti-apoptotic, antioxidant and anti-inflammatory properties of menthol against ethanol-induced gastric ulcers in rats. Wistar rats were orally treated with vehicle, carbenoxolone (100 mg/kg) or menthol (50 mg/kg) and then treated with ethanol to induce gastric ulcers. After euthanasia, stomach samples were prepared for histological slides and biochemical analyses. Immunohistochemical analyses of the cytoprotective and anti-apoptotic heat-shock protein-70 (HSP-70) and the apoptotic Bax protein were performed. The neutrophils were manually counted. The activity of the myeloperoxidase (MPO) was measured. To determine the level of antioxidant functions, the levels of glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and superoxide dismutase (SOD) were measured using ELISA. The levels of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and the anti-inflammatory cytokine interleukin-10 (IL-10) were assessed using ELISA kits. The menthol treated group presented 92% gastroprotection compared to the vehicle-treated group. An increased immunolabeled area was observed for HSP-70, and a decreased immunolabeled area was observed for the Bax protein in the menthol treated group. Menthol treatment induced a decrease in the activity of MPO and SOD, and the protein levels of GSH, GSH-Px and GR were increased. There was also a decrease in the levels of TNF-α and IL-6 and an increase in the level of IL-10. In conclusion, oral treatment with menthol displayed a gastroprotective activity through anti-apoptotic, antixidant and anti-inflammatory mechanisms.
dc.description9
dc.descriptione86686
dc.languageeng
dc.relationPlos One
dc.relationPLoS ONE
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAnti-inflammatory Agents
dc.subjectAntioxidants
dc.subjectApoptosis
dc.subjectEthanol
dc.subjectGastric Mucosa
dc.subjectGlutathione
dc.subjectGlutathione Peroxidase
dc.subjectGlutathione Reductase
dc.subjectHsp70 Heat-shock Proteins
dc.subjectInterleukin-10
dc.subjectInterleukin-6
dc.subjectMale
dc.subjectMenthol
dc.subjectNeutrophils
dc.subjectPeroxidase
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectStomach Ulcer
dc.subjectSuperoxide Dismutase
dc.subjectTumor Necrosis Factor-alpha
dc.subjectBcl-2-associated X Protein
dc.titleThe Gastroprotective Effect Of Menthol: Involvement Of Anti-apoptotic, Antioxidant And Anti-inflammatory Activities.
dc.typeArtículos de revistas


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