Peptides Derived From Phage Display Libraries As Potential Neutralizers Of Shiga Toxin-induced Cytotoxicity In Vitro And In Vivo.

dc.creatorBernedo-Navarro, R A
dc.creatorMiyachiro, M M
dc.creatorda Silva, M J
dc.creatorReis, C F
dc.creatorConceição, R A
dc.creatorGatti, M S V
dc.creatorYano, T
dc.date2014-May
dc.date2015-11-27T13:41:48Z
dc.date2015-11-27T13:41:48Z
dc.date.accessioned2018-03-29T01:19:36Z
dc.date.available2018-03-29T01:19:36Z
dc.identifierJournal Of Applied Microbiology. v. 116, n. 5, p. 1322-33, 2014-May.
dc.identifier1365-2672
dc.identifier10.1111/jam.12451
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24447276
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201101
dc.identifier24447276
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301334
dc.descriptionTo use the phage display technique to develop peptides with the capability to neutralize the cytotoxicity induced by Stx1 and Stx2 toxins produced by Shiga toxin-producing Escherichia coli (STEC). The phage display technique permitted the development of three peptides, named PC7-12, P12-26 and PC7-30, which bind to the globotriaosylceramide (Gb3) receptor for Shiga toxins produced by STEC. Moreover, these peptides were capable of competing efficiently with the Shiga toxins for binding to Gb3. The peptides described herein partially inhibited the Stx-induced cytotoxicity of cell-free filtrates of STEC O157 : H7 and purified Stx toxins in Vero cells. The inhibition of lethality induced by Stx toxins in mice indicated that peptide PC7-30 inhibited the lethality caused by Stx1 (2LD50) in mice. The phage display technique permitted the development of peptides that inhibited the cytotoxicity induced by Stx toxins in vitro. Peptide PC7-30 inhibited the lethality of Stx1 in vivo; this molecule would be a promising candidate for the development of therapeutic agents for STEC-related diseases in humans. The selection of Gb3, the common receptor for Stx1 and Stx2, may contribute to the development of efficient neutralizers for both toxins, and our approach would be an interesting alternative for the development of therapeutic molecules for the treatment of diseases caused by STEC strains.
dc.description116
dc.description1322-33
dc.languageeng
dc.relationJournal Of Applied Microbiology
dc.relationJ. Appl. Microbiol.
dc.rightsfechado
dc.rights© 2014 The Society for Applied Microbiology.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCercopithecus Aethiops
dc.subjectHumans
dc.subjectMice
dc.subjectPeptide Library
dc.subjectPeptides
dc.subjectShiga Toxin 1
dc.subjectShiga Toxin 2
dc.subjectShiga-toxigenic Escherichia Coli
dc.subjectTrihexosylceramides
dc.subjectVero Cells
dc.subjectEhec (enterohaemorrhagic E. coli)
dc.subjectBacteriophages
dc.subjectIntestinal Microbiology
dc.subjectPeptides
dc.subjectToxins
dc.titlePeptides Derived From Phage Display Libraries As Potential Neutralizers Of Shiga Toxin-induced Cytotoxicity In Vitro And In Vivo.
dc.typeArtículos de revistas


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