Fucosylated Chondroitin Sulfate Inhibits Plasmodium Falciparum Cytoadhesion And Merozoite Invasion.

dc.creatorBastos, Marcele F
dc.creatorAlbrecht, Letusa
dc.creatorKozlowski, Eliene O
dc.creatorLopes, Stefanie C P
dc.creatorBlanco, Yara C
dc.creatorCarlos, Bianca C
dc.creatorCastiñeiras, Catarina
dc.creatorVicente, Cristina P
dc.creatorWerneck, Claudio C
dc.creatorWunderlich, Gerhard
dc.creatorFerreira, Marcelo U
dc.creatorMarinho, Claudio R F
dc.creatorMourão, Paulo A S
dc.creatorPavão, Mauro S G
dc.creatorCosta, Fabio T M
dc.date2014
dc.date2015-11-27T13:41:44Z
dc.date2015-11-27T13:41:44Z
dc.date.accessioned2018-03-29T01:19:30Z
dc.date.available2018-03-29T01:19:30Z
dc.identifierAntimicrobial Agents And Chemotherapy. v. 58, n. 4, p. 1862-71, 2014.
dc.identifier1098-6596
dc.identifier10.1128/AAC.00686-13
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24395239
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201073
dc.identifier24395239
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301306
dc.descriptionSequestration of Plasmodium falciparum-infected erythrocytes (Pf-iEs) in the microvasculature of vital organs plays a key role in the pathogenesis of life-threatening malaria complications, such as cerebral malaria and malaria in pregnancy. This phenomenon is marked by the cytoadhesion of Pf-iEs to host receptors on the surfaces of endothelial cells, on noninfected erythrocytes, and in the placental trophoblast; therefore, these sites are potential targets for antiadhesion therapies. In this context, glycosaminoglycans (GAGs), including heparin, have shown the ability to inhibit Pf-iE cytoadherence and growth. Nevertheless, the use of heparin was discontinued due to serious side effects, such as bleeding. Other GAG-based therapies were hampered due to the potential risk of contamination with prions and viruses, as some GAGs are isolated from mammals. In this context, we investigated the effects and mechanism of action of fucosylated chondroitin sulfate (FucCS), a unique and highly sulfated GAG isolated from the sea cucumber, with respect to P. falciparum cytoadhesion and development. FucCS was effective in inhibiting the cytoadherence of Pf-iEs to human lung endothelial cells and placenta cryosections under static and flow conditions. Removal of the sulfated fucose branches of the FucCS structure virtually abolished the inhibitory effects of FucCS. Importantly, FucCS rapidly disrupted rosettes at high levels, and it was also able to block parasite development by interfering with merozoite invasion. Collectively, these findings highlight the potential of FucCS as a candidate for adjunct therapy against severe malaria.
dc.description58
dc.description1862-71
dc.languageeng
dc.relationAntimicrobial Agents And Chemotherapy
dc.relationAntimicrob. Agents Chemother.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAntimalarials
dc.subjectCells, Cultured
dc.subjectChondroitin Sulfates
dc.subjectErythrocytes
dc.subjectHep G2 Cells
dc.subjectHumans
dc.subjectMerozoites
dc.subjectPlasmodium Falciparum
dc.subjectSea Cucumbers
dc.titleFucosylated Chondroitin Sulfate Inhibits Plasmodium Falciparum Cytoadhesion And Merozoite Invasion.
dc.typeArtículos de revistas


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