Lipid Characterization Of Embryo Zones By Silica Plate Laser Desorption Ionization Mass Spectrometry Imaging (sp-ldi-msi).

dc.creatorFerreira, Mônica S
dc.creatorde Oliveira, Diogo N
dc.creatorGonçalves, Roseli F
dc.creatorCatharino, Rodrigo R
dc.date2014-Jan
dc.date2015-11-27T13:41:39Z
dc.date2015-11-27T13:41:39Z
dc.date.accessioned2018-03-29T01:19:24Z
dc.date.available2018-03-29T01:19:24Z
dc.identifierAnalytica Chimica Acta. v. 807, p. 96-102, 2014-Jan.
dc.identifier1873-4324
dc.identifier10.1016/j.aca.2013.11.033
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24356225
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201050
dc.identifier24356225
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301283
dc.descriptionLipid pathways play important biological roles in mammalian embryology, directing early developmental pathways to differentiation. Phospholipids and triglycerides, among others, are the main composing lipids of zona pellucida in several embryo species. Lipid analysis in embryos by mass spectrometry usually requires sample preparation and/or matrix application. This novel approach using silica plate laser desorption/ionization mass spectrometry imaging (SP-LDI-MSI) allows direct single-cell imaging and embryo region discrimination with no matrix coating. Its application is herein described for two- and eight-cell embryos. Lipid biomarkers for blastomere and intact zona pellucida are reported and corroborated by both fragmentation reactions (MS/MS) and images. Results obtained in this work are understood to be of great use for further developments on in vitro bovine fertilization. Since much of the processes can be monitored by characteristic biomarkers, it is now possible to precisely identify cell division errors during early embryo stages, as well as evaluate pre-implantation conditions.
dc.description807
dc.description96-102
dc.languageeng
dc.relationAnalytica Chimica Acta
dc.relationAnal. Chim. Acta
dc.rightsfechado
dc.rightsCopyright © 2013 Elsevier B.V. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBiological Markers
dc.subjectCattle
dc.subjectEmbryo, Mammalian
dc.subjectLipids
dc.subjectPrincipal Component Analysis
dc.subjectSilicon Dioxide
dc.subjectSpectrometry, Mass, Matrix-assisted Laser Desorption-ionization
dc.subject2,5-dihydroxybenzoic Acid
dc.subjectBiomarkers
dc.subjectCer
dc.subjectDhb
dc.subjectDirect Analysis
dc.subjectMaldi-ms
dc.subjectMetabolic Fingerprinting
dc.subjectPa
dc.subjectPca
dc.subjectPe
dc.subjectPl
dc.subjectPrincipal Component Analysis
dc.subjectSp-ldi-msi
dc.subjectSingle-cell
dc.subjectTlc
dc.subjectZp
dc.subjectCeramide
dc.subjectIsok
dc.subjectIsoketals
dc.subjectMatrix-assisted Laser Desorption/ionization Mass Spectrometry
dc.subjectPhosphatidic Acid
dc.subjectPhosphatidylethanolamine
dc.subjectPhospholipid
dc.subjectSilica Plate Laser Desorption/ionization Mass Spectrometry Imaging
dc.subjectThin-layer Chromatography
dc.subjectZona Pellucida
dc.titleLipid Characterization Of Embryo Zones By Silica Plate Laser Desorption Ionization Mass Spectrometry Imaging (sp-ldi-msi).
dc.typeArtículos de revistas


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